Human Recombinant DOT1L protein (V2LY-0526-LY3620)

Name: Human Recombinant DOT1L protein
SKU: V2LY-0526-LY3620
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Protein Construction

This product is Human Recombinant DOT1L protein consist of Amino Acid: 2-416 and predicts a molecular mass of 47.6 kDa.

Molecule Mass

47.6 kDa

Sequence

Amino Acid: 2-416

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

DOT1 Like Histone Lysine Methyltransferase

Function

Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones (PubMed:12123582).

Binds to DNA (PubMed:12628190).

Biological Process

Chromatin silencing at telomere Source: GO_Central
DNA damage checkpoint signaling Source: GO_Central
DNA repair Source: GO_Central
Gene expression Source: Ensembl
Histone H3-K79 methylation Source: MGI
Positive regulation of cell population proliferation Source: MGI
Positive regulation of transcription by RNA polymerase II Source: MGI
Regulation of receptor signaling pathway via JAK-STAT Source: UniProtKB
Regulation of transcription regulatory region DNA binding Source: UniProtKB
Telomere organization Source: BHF-UCL

Cellular Location

Nucleus

Wu, A., Zhi, J., Tian, T., Cihan, A., Cevher, M. A., Liu, Z., ... & Yu, M. (2021). DOT1L complex regulates transcriptional initiation in human erythroleukemic cells. Proceedings of the National Academy of Sciences, 118(27).

Sarno, F., Nebbioso, A., & Altucci, L. (2020). DOT1L: a key target in normal chromatin remodelling and in mixed-lineage leukaemia treatment. Epigenetics, 15(5), 439-453.

Liu, C., Yang, Q., Zhu, Q., Lu, X., Li, M., Hou, T., ... & Zhu, W. G. (2020). CBP mediated DOT1L acetylation confers DOT1L stability and promotes cancer metastasis. Theranostics, 10(4), 1758.

Anderson, C. J., Baird, M. R., Hsu, A., Barbour, E. H., Koyama, Y., Borgnia, M. J., & McGinty, R. K. (2019). Structural basis for recognition of ubiquitylated nucleosome by Dot1L methyltransferase. Cell reports, 26(7), 1681-1690.

Godfrey, L., Crump, N. T., Thorne, R., Lau, I. J., Repapi, E., Dimou, D., ... & Milne, T. A. (2019). DOT1L inhibition reveals a distinct subset of enhancers dependent on H3K79 methylation. Nature communications, 10(1), 1-15.

Worden, E. J., Hoffmann, N. A., Hicks, C. W., & Wolberger, C. (2019). Mechanism of cross-talk between H2B ubiquitination and H3 methylation by Dot1L. Cell, 176(6), 1490-1501.

Wood, K., Tellier, M., & Murphy, S. (2018). DOT1L and H3K79 methylation in transcription and genomic stability. Biomolecules, 8(1), 11.

Zhu, B., Chen, S., Wang, H., Yin, C., Han, C., Peng, C., ... & Cui, R. (2018). The protective role of DOT1L in UV-induced melanomagenesis. Nature communications, 9(1), 1-13.

Stein, E. M., Garcia-Manero, G., Rizzieri, D. A., Tibes, R., Berdeja, J. G., Savona, M. R., ... & Tallman, M. S. (2018). The DOT1L inhibitor pinometostat reduces H3K79 methylation and has modest clinical activity in adult acute leukemia. Blood, The Journal of the American Society of Hematology, 131(24), 2661-2669.

Monteagudo, S., Cornelis, F. M., Aznar-Lopez, C., Yibmantasiri, P., Guns, L. A., Carmeliet, P., ... & Lories, R. J. (2017). DOT1L safeguards cartilage homeostasis and protects against osteoarthritis. Nature communications, 8(1), 1-12.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant DOT1L, Active protein, GST Tag (CAT#: V2LY-0526-LY3621)

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Isotype control

For research use only. Not intended for any clinical use.

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