Mouse Anti-EBAG9 Recombinant Antibody (01) (V2LY-0425-LY1271)

Name: Mouse Anti-EBAG9 Recombinant Antibody (01)
SKU: V2LY-0425-LY1271
Rating: 5 (1 reviews)

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Summary

Host Animal

Mouse

Specificity

Human

Clone

Antibody Isotype

IgG1

Application

IHC, IF

Basic Information

Immunogen

Recombinant full-length human EBAG9 protein.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IF(ICC)	1-2 µg/ml
IHC	1-2 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

BSA & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

estrogen receptor binding site associated antigen 9

Entrez Gene ID

9166

UniProt ID

O00559

Research Area

May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.

Biological Process

Regulation of cell growth Source: UniProtKB

Cellular Location

Golgi apparatus membrane. According to PubMed:10426319, it also exists as a soluble form which has the same biological activities. The existence of such soluble form is however uncertain.

Topology

Extracellular: 1-6
Helical: 7-27
Cytoplasmic: 28-213

More Infomation

References

Wirges, A., Bunse, M., Joedicke, J. J., Blanc, E., Gudipati, V., Moles, M. W., ... & Rehm, A. (2022). EBAG9 silencing exerts an immune checkpoint function without aggravating adverse effects. Molecular Therapy.

Rehm, A., Wirges, A., Hoser, D., Fischer, C., Herda, S., Gerlach, K., ... & Höpken, U. E. (2022). EBAG9 controls CD8+ T cell memory formation responding to tumor challenge in mice. JCI insight, 7(11).

Laaber, K., Dubash, T. D., Melzer, A. M., Christiansen, T., Ha, N., Brors, B., ... & Ball, C. R. (2021). A gene activating in vivo screen identifies EBAG9 as novel metastasis regulator in human colorectal cancer. Cancer Research, 81(13_Supplement), 2852-2852.

Muungo, L. (2020). EBAG9 is a tumor‐promoting and prognostic factor for bladder cancer.

Miyazaki, T., Ikeda, K., Sato, W., Horie-Inoue, K., & Inoue, S. (2018). Extracellular vesicle-mediated EBAG9 transfer from cancer cells to tumor microenvironment promotes immune escape and tumor progression. Oncogenesis, 7(1), 1-11.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Alternative Versions

Mouse Anti-EBAG9 Recombinant Antibody (01)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

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