Human EBAG9 ELISA Kit (V2LY-0626-LY2306)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.28 U/mL
Detection Range
0.5-100 U/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
24.4 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
estrogen receptor binding site associated antigen 9
Research Area
May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.
Biological Process
Regulation of cell growth Source: UniProtKB
Cellular Location
Golgi apparatus membrane. According to PubMed:10426319, it also exists as a soluble form which has the same biological activities. The existence of such soluble form is however uncertain.
Topology
Extracellular: 1-6
Helical: 7-27
Cytoplasmic: 28-213

Wirges, A., Bunse, M., Joedicke, J. J., Blanc, E., Gudipati, V., Moles, M. W., ... & Rehm, A. (2022). EBAG9 silencing exerts an immune checkpoint function without aggravating adverse effects. Molecular Therapy.

Rehm, A., Wirges, A., Hoser, D., Fischer, C., Herda, S., Gerlach, K., ... & Höpken, U. E. (2022). EBAG9 controls CD8+ T cell memory formation responding to tumor challenge in mice. JCI insight, 7(11).

Laaber, K., Dubash, T. D., Melzer, A. M., Christiansen, T., Ha, N., Brors, B., ... & Ball, C. R. (2021). A gene activating in vivo screen identifies EBAG9 as novel metastasis regulator in human colorectal cancer. Cancer Research, 81(13_Supplement), 2852-2852.

Muungo, L. (2020). EBAG9 is a tumor‐promoting and prognostic factor for bladder cancer.

Miyazaki, T., Ikeda, K., Sato, W., Horie-Inoue, K., & Inoue, S. (2018). Extracellular vesicle-mediated EBAG9 transfer from cancer cells to tumor microenvironment promotes immune escape and tumor progression. Oncogenesis, 7(1), 1-11.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant EBAG9 protein, His Tag (CAT#: V2LY-0526-LY3670)

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Isotype control

For research use only. Not intended for any clinical use.

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