Mouse Anti-EBAG9 Recombinant Antibody (CBFYE-0390) (V2LY-0425-LY1270)

Name: Mouse Anti-EBAG9 Recombinant Antibody (CBFYE-0390)
SKU: V2LY-0425-LY1270
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-EBAG9 Recombinant Antibody (CBFYE-0390) (V2LY-0425-LY1270-1) in IF

Indirect immunofluorescence of L1 ORF1p in tet-HeLa cells.
Tet-HeLa cells selected for pTetL1.3 plasmid were treated with 0.5 μg/mL doxycycline for 48 hours to induce L1 expression. At 48 hours, cells were fixed and stained with antibodies against L1 ORF1p and the indicated cellular markers. Alexa Fluor 488 secondary antibodies were used to detect anti-ORF1 antibodies, and Alexa Fluor 594 secondary antibodies were used to detect the cellular markers. DAPI staining shows the nucleus. Shown are representative single planes of confocal imaging. The anti-ORF1p antibody used (JH74 or 4H1) for each subfigure depends on the species source of the cell marker antibody. A. anti-ORF1p and anti-EEA1 (marker for early endosomes). B. anti-ORF1p and anti-RAB7 (marker for late endosomes). C. anti-ORF1 and anti-LAMP2 (marker for lysosomes). D. anti-ORF1 and anti-calnexin (marker for endoplasmic reticulum). E. anti-ORF1 and anti-AIF (marker for mitochondira). F. anti-ORF1 and anti-RCAS1 (marker for golgi). G. anti-ORF1 and anti-EDC4 (marker for P bodies). H. anti-ORF1 and anti-eIF3H (marker for stress granules). ...View More

Horn, A. V., Celic, I., Dong, C., Martirosyan, I., & Han, J. S. (2017). A conserved role for the ESCRT membrane budding complex in LINE retrotransposition. PLoS Genetics, 13(6), e1006837.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0390

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 124-159 within an internal region of human RCAS1.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

estrogen receptor binding site associated antigen 9

Entrez Gene ID

9166

UniProt ID

O00559

Research Area

May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.

Biological Process

Regulation of cell growth Source: UniProtKB

Cellular Location

Golgi apparatus membrane. According to PubMed:10426319, it also exists as a soluble form which has the same biological activities. The existence of such soluble form is however uncertain.

Topology

Extracellular: 1-6
Helical: 7-27
Cytoplasmic: 28-213

Wirges, A., Bunse, M., Joedicke, J. J., Blanc, E., Gudipati, V., Moles, M. W., ... & Rehm, A. (2022). EBAG9 silencing exerts an immune checkpoint function without aggravating adverse effects. Molecular Therapy.

Rehm, A., Wirges, A., Hoser, D., Fischer, C., Herda, S., Gerlach, K., ... & Höpken, U. E. (2022). EBAG9 controls CD8+ T cell memory formation responding to tumor challenge in mice. JCI insight, 7(11).

Laaber, K., Dubash, T. D., Melzer, A. M., Christiansen, T., Ha, N., Brors, B., ... & Ball, C. R. (2021). A gene activating in vivo screen identifies EBAG9 as novel metastasis regulator in human colorectal cancer. Cancer Research, 81(13_Supplement), 2852-2852.

Muungo, L. (2020). EBAG9 is a tumor‐promoting and prognostic factor for bladder cancer.

Miyazaki, T., Ikeda, K., Sato, W., Horie-Inoue, K., & Inoue, S. (2018). Extracellular vesicle-mediated EBAG9 transfer from cancer cells to tumor microenvironment promotes immune escape and tumor progression. Oncogenesis, 7(1), 1-11.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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