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Rabbit Anti-EBAG9 Recombinant Antibody (019) (V2LY-0425-LY1272)

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Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Rabbit
Clone
019
Application
WB, ELISA
Immunogen
Recombinant Human RCAS1 / EBAG9 Protein.
Host Species
Rabbit
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
ELISA1:5,000-1:10,000
WB1:500-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
None
Concentration
Batch dependent
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
estrogen receptor binding site associated antigen 9
Entrez Gene ID
9166
UniProt ID
O00559
Research Area
May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.
Biological Process
Regulation of cell growth Source: UniProtKB
Cellular Location
Golgi apparatus membrane. According to PubMed:10426319, it also exists as a soluble form which has the same biological activities. The existence of such soluble form is however uncertain.
Topology
Extracellular: 1-6
Helical: 7-27
Cytoplasmic: 28-213

Wirges, A., Bunse, M., Joedicke, J. J., Blanc, E., Gudipati, V., Moles, M. W., ... & Rehm, A. (2022). EBAG9 silencing exerts an immune checkpoint function without aggravating adverse effects. Molecular Therapy.

Rehm, A., Wirges, A., Hoser, D., Fischer, C., Herda, S., Gerlach, K., ... & Höpken, U. E. (2022). EBAG9 controls CD8+ T cell memory formation responding to tumor challenge in mice. JCI insight, 7(11).

Laaber, K., Dubash, T. D., Melzer, A. M., Christiansen, T., Ha, N., Brors, B., ... & Ball, C. R. (2021). A gene activating in vivo screen identifies EBAG9 as novel metastasis regulator in human colorectal cancer. Cancer Research, 81(13_Supplement), 2852-2852.

Muungo, L. (2020). EBAG9 is a tumor‐promoting and prognostic factor for bladder cancer.

Miyazaki, T., Ikeda, K., Sato, W., Horie-Inoue, K., & Inoue, S. (2018). Extracellular vesicle-mediated EBAG9 transfer from cancer cells to tumor microenvironment promotes immune escape and tumor progression. Oncogenesis, 7(1), 1-11.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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