Mouse Anti-EFEMP1 Recombinant Antibody (CAP744) (CBMAB-AP2214LY)

Name: Mouse Anti-EFEMP1 Recombinant Antibody (CAP744)
SKU: CBMAB-AP2214LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-EFEMP1 Recombinant Antibody (CAP744) (CBMAB-AP2214LY-1) in WB

Western blotting of individual proteins in bvFTD compared to controls normalized to the housekeeper protein transferrin (Transf), and optical density (OD) measurements of the bands. ...View More

Katzeff, J. S., Bright, F., Lo, K., Kril, J. J., Connolly, A., Crossett, B., ... & Kim, W. S. (2020). Altered serum protein levels in frontotemporal dementia and amyotrophic lateral sclerosis indicate calcium and immunity dysregulation. Scientific reports, 10(1), 13741.

Mouse Anti-EFEMP1 Recombinant Antibody (CAP744) (CBMAB-AP2214LY-2) in IHC

Immunolabeling with antibodies for EFEMP1 of hfRPE cells treated with different doses of C3a or C3a+C3aR antagonist for 2 weeks. Images were taken with confocal. Orthogonal views show the basal deposition of the ECM proteins (left: apical, right: basal). Scale bars 50µm. Average quantification of EFEMP1 fluorescent signal of hfRPE cultures treated with different doses of rhC3a (C3aR-ant) or rhC3a plus C3aR antagonist (C3aR+ant) for 2 weeks. ...View More

Fernandez-Godino, R., & Pierce, E. A. (2018). C3a triggers formation of sub-retinal pigment epithelium deposits via the ubiquitin proteasome pathway. Scientific reports, 8(1), 9679.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CAP744

Application

WB, IP, IF, IHC-P

Immunogen

Amino acids 107-493 of human Fibulin-3.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

EGF Containing Fibulin Extracellular Matrix Protein 1

Introduction

This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.[provided by RefSeq, Nov 2009]

Entrez Gene ID

Human	2202
Mouse	216616
Rat	305604

UniProt ID

Human	Q12805
Mouse	Q8BPB5
Rat	O35568

Alternative Names

EGF Containing Fibulin Extracellular Matrix Protein 1; EGF Containing Fibulin Like Extracellular Matrix Protein 1; EGF-Containing Fibulin-Like Extracellular Matrix Protein 1; Extracellular Protein S1-5; Fibulin-3; FIBL-3; FBLN3; FBNL; Fibrillin-Like Protein;

Research Area

Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth.

Biological Process

Camera-type eye development Source: UniProtKB
Embryonic eye morphogenesis Source: UniProtKB
Epidermal growth factor receptor signaling pathway Source: UniProtKB
Negative regulation of chondrocyte differentiation Source: UniProtKB
Peptidyl-tyrosine phosphorylation Source: UniProtKB
Post-embryonic eye morphogenesis Source: UniProtKB
Regulation of transcription, DNA-templated Source: UniProtKB
Visual perception Source: ProtInc

Cellular Location

Extracellular space; Extracellular matrix. Localizes to the lamina propria underneath the olfactory epithelium.

Involvement in disease

Doyne honeycomb retinal dystrophy (DHRD):
Autosomal dominant disease characterized by yellow-white deposits known as drusen that accumulate beneath the retinal pigment epithelium.

Collantes, E. R. A., Delfin, M. S., Fan, B., Torregosa, J. M. R., Siguan‐Bell, C., Florcruz, N. V. D. G., ... & Wiggs, J. L. (2022). EFEMP1 rare variants cause familial juvenile‐onset open‐angle glaucoma. Human mutation, 43(2), 240-252.

McGrath, E. R., Himali, J. J., Levy, D., Yang, Q., DeCarli, C. S., Courchesne, P., ... & Seshadri, S. (2022). Plasma EFEMP1 is associated with brain aging and dementia: The Framingham Heart Study. Journal of Alzheimer's Disease, (Preprint), 1-10.

Peng, X., Guo, Z., Zhang, Y., Sun, B., & Zhang, Q. (2022). EFEMP1 in direct inguinal hernia: correlation with TIMP3 and regulation toward elastin homoeostasis as well as fibroblast mobility. Journal of Investigative Surgery, 35(1), 203-211.

Garland, D. L., Pierce, E. A., & Fernandez-Godino, R. (2021). Complement C5 is not critical for the formation of sub-RPE deposits in Efemp1 mutant mice. Scientific Reports, 11(1), 1-11.

Ying, X., Liu, B., Yuan, Z., Huang, Y., Chen, C., Jiang, X., ... & Ji, W. (2021). METTL1‐m7G‐EGFR/EFEMP1 axis promotes the bladder cancer development. Clinical and translational medicine, 11(12), e675.

Cheng, L., Chen, C., Guo, W., Liu, K., Zhao, Q., Lu, P., ... & Xu, X. (2021). EFEMP1 Overexpression Contributes to Neovascularization in Age-Related Macular Degeneration. Frontiers in Pharmacology, 11, 547436.

Chen, T. J., Chan, T. C., Li, W. S., Li, C. F., Ke, H. L., Wei, Y. C., ... & Li, W. M. (2021). Utility of EFEMP1 in the prediction of oncologic outcomes of urothelial carcinoma. Genes, 12(6), 872.

Driver, S. G., Jackson, M. R., Richter, K., Tomlinson, P., Brockway, B., Halliday, B. J., ... & Wade, E. M. (2020). Biallelic variants in EFEMP1 in a man with a pronounced connective tissue phenotype. European Journal of Human Genetics, 28(4), 445-452.

Wang, Z., Kang, J., Lian, J., Huang, L., Xie, W., Zhao, D., ... & Lin, Z. (2020). EFEMP1 as a potential biomarker for diagnosis and prognosis of osteosarcoma. BioMed research international, 2020.

Cosentino, G., Romero-Cordoba, S., Plantamura, I., Cataldo, A., & Iorio, M. V. (2020). miR-9-mediated inhibition of EFEMP1 contributes to the acquisition of pro-tumoral properties in normal fibroblasts. Cells, 9(9), 2143.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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