Mouse Efemp1 ELISA Kit (V2LY-0626-LY5883)

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Tested Data
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0099 ng/mL
Detection Range
0.02-4.5 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
55.0 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
EGF Containing Fibulin Extracellular Matrix Protein 1
Research Area
Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth.
Biological Process
Camera-type eye development Source: UniProtKB
Embryonic eye morphogenesis Source: UniProtKB
Epidermal growth factor receptor signaling pathway Source: UniProtKB
Negative regulation of chondrocyte differentiation Source: UniProtKB
Peptidyl-tyrosine phosphorylation Source: UniProtKB
Post-embryonic eye morphogenesis Source: UniProtKB
Regulation of transcription, DNA-templated Source: UniProtKB
Visual perception Source: ProtInc
Cellular Location
Extracellular space; Extracellular matrix. Localizes to the lamina propria underneath the olfactory epithelium.
Involvement in disease
Doyne honeycomb retinal dystrophy (DHRD):
Autosomal dominant disease characterized by yellow-white deposits known as drusen that accumulate beneath the retinal pigment epithelium.

Collantes, E. R. A., Delfin, M. S., Fan, B., Torregosa, J. M. R., Siguan‐Bell, C., Florcruz, N. V. D. G., ... & Wiggs, J. L. (2022). EFEMP1 rare variants cause familial juvenile‐onset open‐angle glaucoma. Human mutation, 43(2), 240-252.

McGrath, E. R., Himali, J. J., Levy, D., Yang, Q., DeCarli, C. S., Courchesne, P., ... & Seshadri, S. (2022). Plasma EFEMP1 is associated with brain aging and dementia: The Framingham Heart Study. Journal of Alzheimer's Disease, (Preprint), 1-10.

Peng, X., Guo, Z., Zhang, Y., Sun, B., & Zhang, Q. (2022). EFEMP1 in direct inguinal hernia: correlation with TIMP3 and regulation toward elastin homoeostasis as well as fibroblast mobility. Journal of Investigative Surgery, 35(1), 203-211.

Garland, D. L., Pierce, E. A., & Fernandez-Godino, R. (2021). Complement C5 is not critical for the formation of sub-RPE deposits in Efemp1 mutant mice. Scientific Reports, 11(1), 1-11.

Ying, X., Liu, B., Yuan, Z., Huang, Y., Chen, C., Jiang, X., ... & Ji, W. (2021). METTL1‐m7G‐EGFR/EFEMP1 axis promotes the bladder cancer development. Clinical and translational medicine, 11(12), e675.

Cheng, L., Chen, C., Guo, W., Liu, K., Zhao, Q., Lu, P., ... & Xu, X. (2021). EFEMP1 Overexpression Contributes to Neovascularization in Age-Related Macular Degeneration. Frontiers in Pharmacology, 11, 547436.

Chen, T. J., Chan, T. C., Li, W. S., Li, C. F., Ke, H. L., Wei, Y. C., ... & Li, W. M. (2021). Utility of EFEMP1 in the prediction of oncologic outcomes of urothelial carcinoma. Genes, 12(6), 872.

Driver, S. G., Jackson, M. R., Richter, K., Tomlinson, P., Brockway, B., Halliday, B. J., ... & Wade, E. M. (2020). Biallelic variants in EFEMP1 in a man with a pronounced connective tissue phenotype. European Journal of Human Genetics, 28(4), 445-452.

Wang, Z., Kang, J., Lian, J., Huang, L., Xie, W., Zhao, D., ... & Lin, Z. (2020). EFEMP1 as a potential biomarker for diagnosis and prognosis of osteosarcoma. BioMed research international, 2020.

Cosentino, G., Romero-Cordoba, S., Plantamura, I., Cataldo, A., & Iorio, M. V. (2020). miR-9-mediated inhibition of EFEMP1 contributes to the acquisition of pro-tumoral properties in normal fibroblasts. Cells, 9(9), 2143.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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