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Mouse Anti-EID1 Recombinant Antibody (CBFYE-0630) (V2LY-0425-LY1621)

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Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Mouse
Clone
CBFYE-0630
Application
WB, IF
Immunogen
Synthetic peptide containing amino acids 1-19 of human EID1 protein.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1 μg/ml
IF(ICC)1 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Glycerol & PBS
Preservative
None
Concentration
1 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
EP300 Interacting Inhibitor Of Differentiation 1
Entrez Gene ID
23741
UniProt ID
Q9Y6B2
Research Area
Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms.
Biological Process
Cell cycle Source: UniProtKB-KW
Cell differentiation Source: UniProtKB
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: MGI
Regulation of cell differentiation Source: InterPro
Cellular Location
Cytoplasm; Nucleus. May shuttle between nucleus and cytoplasm.
PTM
Ubiquitinated in U2OS osteosarcoma cells and is rapidly degraded by proteasome as cells exit the cell cycle exit.

Li, Y., Feng, J., Bian, Y., Cheng, W., & Qiao, C. (2022). EID1 plays a protective role in early-onset pre-eclampsia via promoting proliferation and invasion in trophoblast cells. Folia Histochemica et Cytobiologica, 60(1), 31-43.

Fu, X., Ding, B., Wang, C., Chen, C., Wang, J., Fei, X., & Xu, R. (2019). EID1 plays a crucial role in proliferation of neural stem cell. Biochemical and biophysical research communications, 512(4), 763-769.

Fu, X., Luo, L., Yi, R., Ding, B., Wang, C., Zhang, W., ... & Xu, R. (2019). Transcriptome profiling in Eid1-KO mice brain shows that Eid1 links cell proliferation in the brain. Gene, 717, 143998.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-EID1 Recombinant Antibody (CBFYE-0630) (CAT#: CBMAB-E1036-FY)

Related Products

Rabbit Anti-EID1 Recombinant Antibody (EG1057) (CAT#: CBMAB-EN1247-LY)

Mouse Anti-EID1 Recombinant Antibody (EG1057) (CAT#: V2LY-0425-LY1622)

Mouse Anti-EID1 Recombinant Antibody (CBFYE-0630) (CAT#: CBMAB-E1036-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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