Mouse Anti-GAK Recombinant Antibody (4C10) (V2LY-1225-LY604)

Name: Mouse Anti-GAK Recombinant Antibody (4C10)
SKU: V2LY-1225-LY604
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-GAK Recombinant Antibody (4C10) (V2LY-1225-LY604) in WB

HEK293 cells were transfected with siRNAs GAK1 or NT. GAK expression level was determined by Western blotting. ...View More

Wang, C., Wang, J., Shuai, L., Ma, X., Zhang, H., Liu, R., Chen, W., Wang, X., Ge, J., Wen, Z., & Bu, Z. (2019). The Serine/Threonine Kinase AP2-Associated Kinase 1 Plays an Important Role in Rabies Virus Entry. Viruses, 12(1), 45.

Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

4C10

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 1-360 of rat GAK.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

cyclin G associated kinase

Entrez Gene ID

Human	2580
Mouse	231580
Rat	81659

UniProt ID

Human	O14976
Mouse	Q99KY4
Rat	P97874

Function

Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.

Biological Process

Cell cycle Source: UniProtKB-KW
Chaperone cofactor-dependent protein refolding Source: BHF-UCL
Clathrin coat disassembly Source: ParkinsonsUK-UCL
Clathrin-coated pit assembly Source: ParkinsonsUK-UCL
Clathrin-dependent endocytosis Source: ParkinsonsUK-UCL
Endoplasmic reticulum organization Source: ParkinsonsUK-UCL
Golgi organization Source: ParkinsonsUK-UCL
Golgi to lysosome transport Source: ParkinsonsUK-UCL
Negative regulation of neuron projection development Source: ParkinsonsUK-UCL
Protein localization to Golgi apparatus Source: ParkinsonsUK-UCL
Protein localization to plasma membrane Source: ParkinsonsUK-UCL
Receptor-mediated endocytosis Source: ParkinsonsUK-UCL
Synaptic vesicle uncoating Source: BHF-UCL

Cellular Location

Trans-Golgi network; Perinuclear region; Focal adhesion. Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-GAK Recombinant Antibody (4C10) (CAT#: CBMAB-G1831-LY)

Antibody Pairs

GAK Matched Antibody Pair (461) (CAT#: APMAB-461LY)

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GAK Matched Antibody Pair (461) (CAT#: APMAB-461LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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