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Rabbit Anti-GAK Recombinant Antibody (EG1279) (V2LY-1225-LY607)

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Datasheet
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Basic Information

Host Animal
Rabbit
Clone
EG1279
Application
WB, IP
Immunogen
Synthetic peptide corresponding to residues surrounding Pro758 of human GAK protein.
Host Species
Rabbit
Specificity
Human, Mouse, Rat, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
ApplicationNote
WB1:1,000
IP 1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
HEPES, BSA, glycerol
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
cyclin G associated kinase
Entrez Gene ID
Human2580
Mouse231580
Rat81659
UniProt ID
HumanO14976
MouseQ99KY4
RatP97874
Function
Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.
Biological Process
Cell cycle Source: UniProtKB-KW
Chaperone cofactor-dependent protein refolding Source: BHF-UCL
Clathrin coat disassembly Source: ParkinsonsUK-UCL
Clathrin-coated pit assembly Source: ParkinsonsUK-UCL
Clathrin-dependent endocytosis Source: ParkinsonsUK-UCL
Endoplasmic reticulum organization Source: ParkinsonsUK-UCL
Golgi organization Source: ParkinsonsUK-UCL
Golgi to lysosome transport Source: ParkinsonsUK-UCL
Negative regulation of neuron projection development Source: ParkinsonsUK-UCL
Protein localization to Golgi apparatus Source: ParkinsonsUK-UCL
Protein localization to plasma membrane Source: ParkinsonsUK-UCL
Receptor-mediated endocytosis Source: ParkinsonsUK-UCL
Synaptic vesicle uncoating Source: BHF-UCL
Cellular Location
Trans-Golgi network; Perinuclear region; Focal adhesion. Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rabbit Anti-GAK Recombinant Antibody (EG1279) (CAT#: CBMAB-EN1543-LY)

Antibody Pairs

GAK Matched Antibody Pair (461) (CAT#: APMAB-461LY)

Related Products

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Mouse Anti-GAK Recombinant Antibody (6) (CAT#: CBMAB-G0149-LY)

GAK Matched Antibody Pair (461) (CAT#: APMAB-461LY)

Mouse Anti-GAK Recombinant Antibody (1C2) (CAT#: CBMAB-G1830-LY)

Rabbit Anti-GAK Recombinant Antibody (EG1279) (CAT#: CBMAB-EN1543-LY)

Mouse Anti-GAK Recombinant Antibody (1C2) (CAT#: V2LY-1225-LY605)

Mouse Anti-GAK Recombinant Antibody (4C10) (CAT#: V2LY-1225-LY604)

Mouse Anti-GAK Recombinant Antibody (4C10) (CAT#: CBMAB-G1831-LY)

Mouse Anti-GAK Recombinant Antibody (CBFYH-3422) (CAT#: CBMAB-H3968-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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