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Mouse Anti-GRK6 Recombinant Antibody (2G1) (CBMAB-G5014-LY)

This product is antibody recognizes GRK6. The antibody 2G1 immunoassay techniques such as: ELISA, WB.
See all GRK6 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
2G1
Antibody Isotype
IgG1, κ
Application
ELISA, WB

Basic Information

Immunogen
Recombinant protein corresponding to aa71-170 from human GRK6 with GST tag. MW of the GST tag alone is 26kD
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
G protein-coupled receptor kinase 6
Introduction
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human2870
Mouse26385
Rat59076
UniProt ID
HumanP43250
MouseO70293
RatP97711
Alternative Names
G Protein-Coupled Receptor Kinase 6; EC 2.7.11.16; GPRK6; G Protein-Coupled Receptor Kinase GRK6; EC 2.7.11;
Function
Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (By similarity).

Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro).
Biological Process
G protein-coupled receptor signaling pathway Source: Reactome
Protein phosphorylation Source: GO_Central
Regulation of G protein-coupled receptor signaling pathway Source: ProtInc
Regulation of signal transduction Source: GO_Central
Wnt signaling pathway Source: UniProtKB-KW
Cellular Location
Membrane
PTM
It is uncertain whether palmitoylation is on Cys-561 and/or Cys-562 and/or Cys-565.

Varney, M. J., Steyaert, W., Coucke, P. J., Delanghe, J. R., Uehling, D. E., Joseph, B., ... & Benovic, J. L. (2022). G protein–coupled receptor kinase 6 (GRK6) regulates insulin processing and secretion via effects on proinsulin conversion to insulin. Journal of Biological Chemistry, 298(10).

Uehling, D. E., Joseph, B., Chung, K. C., Zhang, A. X., Ler, S., Prakesch, M. A., ... & Tiedemann, R. E. (2021). Design, synthesis, and characterization of 4-aminoquinazolines as potent inhibitors of the G protein-coupled receptor kinase 6 (GRK6) for the treatment of multiple myeloma. Journal of medicinal chemistry, 64(15), 11129-11147.

Olson, T. L., Zhang, S., Labban, D., Kaschner, E., Aceves, M., Iyer, S., ... & Fromme, P. (2021). Protein expression and purification of G-protein coupled receptor kinase 6 (GRK6), toward structure-based drug design and discovery for multiple myeloma. Protein Expression and Purification, 185, 105890.

Stegen, M., Engler, A., Ochsenfarth, C., Manthey, I., Peters, J., Siffert, W., & Frey, U. H. (2021). Characterization of the G protein-coupled receptor kinase 6 promoter reveals a functional CREB binding site. Plos one, 16(2), e0247087.

Chaudhary, P. K., Kim, S., Jee, Y., Lee, S. H., Park, K. M., & Kim, S. (2020). Role of GRK6 in the regulation of platelet activation through selective G protein-coupled receptor (GPCR) desensitization. International Journal of Molecular Sciences, 21(11), 3932.

Wu, W., Sung, C. C., Yu, P., Li, J., & Chung, K. K. (2020). S-Nitrosylation of G protein-coupled receptor kinase 6 and Casein kinase 2 alpha modulates their kinase activity toward alpha-synuclein phosphorylation in an animal model of Parkinson’s disease. PLoS One, 15(4), e0232019.

Serafin, D. S., Allyn, B., Sassano, M. F., Timoshchenko, R. G., Mattox, D., Brozowski, J. M., ... & Billard, M. J. (2019). Chemerin-activated functions of CMKLR1 are regulated by G protein-coupled receptor kinase 6 (GRK6) and β-arrestin 2 in inflammatory macrophages. Molecular immunology, 106, 12-21.

Yao, S., Wu, D., Chen, J., Wang, P., Lv, X., & Huang, J. (2019). Hypermethylation of the G protein‐coupled receptor kinase 6 (GRK6) promoter inhibits binding of C/EBPα, and GRK6 knockdown promotes cell migration and invasion in lung adenocarcinoma cells. FEBS Open Bio, 9(4), 605-617.

Che, X., Zhang, G., Zhang, X., & Xue, J. (2018). Overexpression of G protein-coupled receptor kinase 6 (GRK6) is associated with progression and poor prognosis of papillary thyroid carcinoma. Medical science monitor: international medical journal of experimental and clinical research, 24, 3540.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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