Rabbit Anti-GRK6 Recombinant Antibody (EG1447) (CBMAB-EN1728-LY)

Name: Rabbit Anti-GRK6 Recombinant Antibody (EG1447)
SKU: CBMAB-EN1728-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

EG1447

Application

Immunogen

Human GRK6.

Specificity

Human, Mouse

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, BSA, glycerol

Preservative

Sodium azide

Concentration

1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

G protein-coupled receptor kinase 6

Introduction

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene.

Entrez Gene ID

Human	2870
Mouse	26385

UniProt ID

Human	P43250
Mouse	O70293

Alternative Names

GPRK6

Biological Process

G protein-coupled receptor signaling pathway Source: Reactome
Protein phosphorylation Source: GO_Central
Regulation of G protein-coupled receptor signaling pathway Source: ProtInc
Regulation of signal transduction Source: GO_Central
Wnt signaling pathway Source: UniProtKB-KW

Cellular Location

Membrane

PTM

It is uncertain whether palmitoylation is on Cys-561 and/or Cys-562 and/or Cys-565.

Varney, M. J., Steyaert, W., Coucke, P. J., Delanghe, J. R., Uehling, D. E., Joseph, B., ... & Benovic, J. L. (2022). G protein–coupled receptor kinase 6 (GRK6) regulates insulin processing and secretion via effects on proinsulin conversion to insulin. Journal of Biological Chemistry, 298(10).

Uehling, D. E., Joseph, B., Chung, K. C., Zhang, A. X., Ler, S., Prakesch, M. A., ... & Tiedemann, R. E. (2021). Design, synthesis, and characterization of 4-aminoquinazolines as potent inhibitors of the G protein-coupled receptor kinase 6 (GRK6) for the treatment of multiple myeloma. Journal of medicinal chemistry, 64(15), 11129-11147.

Olson, T. L., Zhang, S., Labban, D., Kaschner, E., Aceves, M., Iyer, S., ... & Fromme, P. (2021). Protein expression and purification of G-protein coupled receptor kinase 6 (GRK6), toward structure-based drug design and discovery for multiple myeloma. Protein Expression and Purification, 185, 105890.

Stegen, M., Engler, A., Ochsenfarth, C., Manthey, I., Peters, J., Siffert, W., & Frey, U. H. (2021). Characterization of the G protein-coupled receptor kinase 6 promoter reveals a functional CREB binding site. Plos one, 16(2), e0247087.

Chaudhary, P. K., Kim, S., Jee, Y., Lee, S. H., Park, K. M., & Kim, S. (2020). Role of GRK6 in the regulation of platelet activation through selective G protein-coupled receptor (GPCR) desensitization. International Journal of Molecular Sciences, 21(11), 3932.

Wu, W., Sung, C. C., Yu, P., Li, J., & Chung, K. K. (2020). S-Nitrosylation of G protein-coupled receptor kinase 6 and Casein kinase 2 alpha modulates their kinase activity toward alpha-synuclein phosphorylation in an animal model of Parkinson’s disease. PLoS One, 15(4), e0232019.

Yao, S., Wu, D., Chen, J., Wang, P., Lv, X., & Huang, J. (2019). Hypermethylation of the G protein‐coupled receptor kinase 6 (GRK6) promoter inhibits binding of C/EBPα, and GRK6 knockdown promotes cell migration and invasion in lung adenocarcinoma cells. FEBS Open Bio, 9(4), 605-617.

Che, X., Zhang, G., Zhang, X., & Xue, J. (2018). Overexpression of G protein-coupled receptor kinase 6 (GRK6) is associated with progression and poor prognosis of papillary thyroid carcinoma. Medical science monitor: international medical journal of experimental and clinical research, 24, 3540.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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