Mouse Anti-HSD3B1 (AA 1-374) Monoclonal Antibody (CBFYH-3208) (CBMAB-H3032-FY)

Name: Mouse Anti-HSD3B1 (AA 1-374) Monoclonal Antibody (CBFYH-3208)
SKU: CBMAB-H3032-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYH-3208

Application

WB, IHC

Immunogen

Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Concentration

0.5 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 1-374

More Infomation

Target

Full Name

Hydroxy-Delta-5-Steroid Dehydrogenase, 3 Beta- And Steroid Delta-Isomerase 1

Introduction

The protein encoded by this gene is an enzyme that catalyzes the oxidative conversion of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, which leads to the production of all classes of steroid hormones. The encoded protein also catalyzes the interconversion of 3-beta-hydroxy- and 3-keto-5-alpha-androstane steroids.

Entrez Gene ID

3283

UniProt ID

P14060

Alternative Names

Hydroxy-Delta-5-Steroid Dehydrogenase, 3 Beta- And Steroid Delta-Isomerase 1; 3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type I; Short Chain Dehydrogenase/Reductase Family 11E, Member 1; Trophoblast Antigen FDO161G; 3-Beta-HSD I; HSDB3A; 3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type 1; 3-Beta-Hydroxy-Delta(5)-Steroid Dehydrogenase; 3-Beta-Hydroxy-5-Ene Steroid Dehydrogenase; Delta-5-3-Ketosteroid Isomerase

Function

A bifunctional enzyme responsible for the oxidation and isomerization of 3beta-hydroxy-Delta5-steroid precursors to 3-oxo-Delta4-steroids, an essential step in steroid hormone biosynthesis. Specifically catalyzes the conversion of pregnenolone to progesterone, 17alpha-hydroxypregnenolone to 17alpha-hydroxyprogesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone. Additionally, catalyzes the interconversion between 3beta-hydroxy and 3-oxo-5alpha-androstane steroids controlling the bioavalability of the active forms. Specifically converts dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Also converts androstanedione, a precursor of testosterone and estrone, to epiandrosterone (PubMed:1401999, PubMed:2139411).

Expected to use NAD+ as preferred electron donor for the 3beta-hydroxy-steroid dehydrogenase activity and NADPH for the 3-ketosteroid reductase activity (Probable).

Biological Process

Androgen biosynthetic process Source: UniProtKB
C21-steroid hormone metabolic process Source: GO_Central
Estrogen biosynthetic process Source: UniProtKB
Hippocampus development Source: GO_Central
Response to corticosterone Source: GO_Central
Steroid biosynthetic process Source: UniProtKB

Cellular Location

Mitochondrion membrane; Endoplasmic reticulum membrane

Topology

Helical: 288-308

Cui, D., Li, J., Zhu, Z., Berk, M., Hardaway, A., McManus, J., ... & Sharifi, N. (2023). Cancer-associated fibroblast-secreted glucosamine alters the androgen biosynthesis program in prostate cancer via HSD3B1 upregulation. The Journal of Clinical Investigation, 133(7).

Kruse, M. L., Patel, M., McManus, J., Chung, Y. M., Li, X., Wei, W., ... & Sharifi, N. (2021). Adrenal-permissive HSD3B1 genetic inheritance and risk of estrogen-driven postmenopausal breast cancer. JCI insight, 6(20).

Zein, J., Gaston, B., Bazeley, P., DeBoer, M. D., Igo Jr, R. P., Bleecker, E. R., ... & Sharifi, N. (2020). HSD3B1 genotype identifies glucocorticoid responsiveness in severe asthma. Proceedings of the National Academy of Sciences, 117(4), 2187-2193.

Thomas, L., & Sharifi, N. (2020). Germline HSD3B1 genetics and prostate cancer outcomes. Urology, 145, 13-21.

Hearn, J. W., Sweeney, C. J., Almassi, N., Reichard, C. A., Reddy, C. A., Li, H., ... & Sharifi, N. (2020). HSD3B1 genotype and clinical outcomes in metastatic castration-sensitive prostate cancer. JAMA oncology, 6(4), e196496-e196496.

Sabharwal, N., & Sharifi, N. (2019). HSD3B1 genotypes conferring adrenal-restrictive and adrenal-permissive phenotypes in prostate cancer and beyond. Endocrinology, 160(9), 2180-2188.

Alyamani, M., Emamekhoo, H., Park, S., Taylor, J., Almassi, N., Upadhyay, S., ... & Sharifi, N. (2018). HSD3B1 (1245A> C) variant regulates dueling abiraterone metabolite effects in prostate cancer. The Journal of Clinical Investigation, 128(8), 3333-3340.

Hettel, D., & Sharifi, N. (2018). HSD3B1 status as a biomarker of androgen deprivation resistance and implications for prostate cancer. Nature Reviews Urology, 15(3), 191-196.

Almassi, N., Reichard, C., Li, J., Russell, C., Perry, J., Ryan, C. J., ... & Sharifi, N. (2018). HSD3B1 and response to a nonsteroidal CYP17A1 inhibitor in castration-resistant prostate cancer. JAMA oncology, 4(4), 554-557.

Hearn, J. W., Xie, W., Nakabayashi, M., Almassi, N., Reichard, C. A., Pomerantz, M., ... & Sharifi, N. (2018). Association of HSD3B1 genotype with response to androgen-deprivation therapy for biochemical recurrence after radiotherapy for localized prostate cancer. JAMA oncology, 4(4), 558-562.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

HSD3B1 Matched Antibody Pair (574) (CAT#: APMAB-574LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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