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Mouse Anti-HSD3B1 Recombinant Antibody (3C11-D4) (CBMAB-A4103-LY)

The product is antibody recognizes HSD3B1. The antibody 3C11-D4 immunoassay techniques such as: WB, ELISA.
See all HSD3B1 antibodies
Published Data
Fig.1 HSD3B1 immunoreactivity in normal early placentas.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
3C11-D4
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
HSD3B1 (AAH31999.1, 1 a.a. ~ 373 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Hydroxy-Delta-5-Steroid Dehydrogenase, 3 Beta- And Steroid Delta-Isomerase 1
Entrez Gene ID
3283
UniProt ID
P14060
Alternative Names
HSD3B; HSDB3; SDR11E1
Function
A bifunctional enzyme responsible for the oxidation and isomerization of 3beta-hydroxy-Delta5-steroid precursors to 3-oxo-Delta4-steroids, an essential step in steroid hormone biosynthesis. Specifically catalyzes the conversion of pregnenolone to progesterone, 17alpha-hydroxypregnenolone to 17alpha-hydroxyprogesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone. Additionally, catalyzes the interconversion between 3beta-hydroxy and 3-oxo-5alpha-androstane steroids controlling the bioavalability of the active forms. Specifically converts dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Also converts androstanedione, a precursor of testosterone and estrone, to epiandrosterone (PubMed:1401999, PubMed:2139411).

Expected to use NAD+ as preferred electron donor for the 3beta-hydroxy-steroid dehydrogenase activity and NADPH for the 3-ketosteroid reductase activity (Probable).
Biological Process
Androgen biosynthetic process Source: UniProtKB
C21-steroid hormone metabolic process Source: GO_Central
Estrogen biosynthetic process Source: UniProtKB
Hippocampus development Source: GO_Central
Response to corticosterone Source: GO_Central
Steroid biosynthetic process Source: UniProtKB
Cellular Location
Mitochondrion membrane; Endoplasmic reticulum membrane
Topology
Helical: 288-308

Cui, D., Li, J., Zhu, Z., Berk, M., Hardaway, A., McManus, J., ... & Sharifi, N. (2023). Cancer-associated fibroblast-secreted glucosamine alters the androgen biosynthesis program in prostate cancer via HSD3B1 upregulation. The Journal of Clinical Investigation, 133(7).

Kruse, M. L., Patel, M., McManus, J., Chung, Y. M., Li, X., Wei, W., ... & Sharifi, N. (2021). Adrenal-permissive HSD3B1 genetic inheritance and risk of estrogen-driven postmenopausal breast cancer. JCI insight, 6(20).

Zein, J., Gaston, B., Bazeley, P., DeBoer, M. D., Igo Jr, R. P., Bleecker, E. R., ... & Sharifi, N. (2020). HSD3B1 genotype identifies glucocorticoid responsiveness in severe asthma. Proceedings of the National Academy of Sciences, 117(4), 2187-2193.

Thomas, L., & Sharifi, N. (2020). Germline HSD3B1 genetics and prostate cancer outcomes. Urology, 145, 13-21.

Hearn, J. W., Sweeney, C. J., Almassi, N., Reichard, C. A., Reddy, C. A., Li, H., ... & Sharifi, N. (2020). HSD3B1 genotype and clinical outcomes in metastatic castration-sensitive prostate cancer. JAMA oncology, 6(4), e196496-e196496.

Sabharwal, N., & Sharifi, N. (2019). HSD3B1 genotypes conferring adrenal-restrictive and adrenal-permissive phenotypes in prostate cancer and beyond. Endocrinology, 160(9), 2180-2188.

Alyamani, M., Emamekhoo, H., Park, S., Taylor, J., Almassi, N., Upadhyay, S., ... & Sharifi, N. (2018). HSD3B1 (1245A> C) variant regulates dueling abiraterone metabolite effects in prostate cancer. The Journal of Clinical Investigation, 128(8), 3333-3340.

Hettel, D., & Sharifi, N. (2018). HSD3B1 status as a biomarker of androgen deprivation resistance and implications for prostate cancer. Nature Reviews Urology, 15(3), 191-196.

Almassi, N., Reichard, C., Li, J., Russell, C., Perry, J., Ryan, C. J., ... & Sharifi, N. (2018). HSD3B1 and response to a nonsteroidal CYP17A1 inhibitor in castration-resistant prostate cancer. JAMA oncology, 4(4), 554-557.

Hearn, J. W., Xie, W., Nakabayashi, M., Almassi, N., Reichard, C. A., Pomerantz, M., ... & Sharifi, N. (2018). Association of HSD3B1 genotype with response to androgen-deprivation therapy for biochemical recurrence after radiotherapy for localized prostate cancer. JAMA oncology, 4(4), 558-562.

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For research use only. Not intended for any clinical use.

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