Rat Hsd3b1 ELISA Kit (V2LY-0626-LY1651)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.021 ng/mL
Detection Range
0.05-20 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Rat
Assay Type
Sandwich
Reactivity
Rat
Assay Time
1.5 h
Molecule Mass
42.0 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Hydroxy-Delta-5-Steroid Dehydrogenase, 3 Beta- And Steroid Delta-Isomerase 1
Function
A bifunctional enzyme responsible for the oxidation and isomerization of 3beta-hydroxy-Delta5-steroid precursors to 3-oxo-Delta4-steroids, an essential step in steroid hormone biosynthesis. Specifically catalyzes the conversion of pregnenolone to progesterone, 17alpha-hydroxypregnenolone to 17alpha-hydroxyprogesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone. Additionally, catalyzes the interconversion between 3beta-hydroxy and 3-oxo-5alpha-androstane steroids controlling the bioavalability of the active forms. Specifically converts dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Also converts androstanedione, a precursor of testosterone and estrone, to epiandrosterone (PubMed:1401999, PubMed:2139411).

Expected to use NAD+ as preferred electron donor for the 3beta-hydroxy-steroid dehydrogenase activity and NADPH for the 3-ketosteroid reductase activity (Probable).
Biological Process
Androgen biosynthetic process Source: UniProtKB
C21-steroid hormone metabolic process Source: GO_Central
Estrogen biosynthetic process Source: UniProtKB
Hippocampus development Source: GO_Central
Response to corticosterone Source: GO_Central
Steroid biosynthetic process Source: UniProtKB
Cellular Location
Mitochondrion membrane; Endoplasmic reticulum membrane
Topology
Helical: 288-308

Cui, D., Li, J., Zhu, Z., Berk, M., Hardaway, A., McManus, J., ... & Sharifi, N. (2023). Cancer-associated fibroblast-secreted glucosamine alters the androgen biosynthesis program in prostate cancer via HSD3B1 upregulation. The Journal of Clinical Investigation, 133(7).

Kruse, M. L., Patel, M., McManus, J., Chung, Y. M., Li, X., Wei, W., ... & Sharifi, N. (2021). Adrenal-permissive HSD3B1 genetic inheritance and risk of estrogen-driven postmenopausal breast cancer. JCI insight, 6(20).

Zein, J., Gaston, B., Bazeley, P., DeBoer, M. D., Igo Jr, R. P., Bleecker, E. R., ... & Sharifi, N. (2020). HSD3B1 genotype identifies glucocorticoid responsiveness in severe asthma. Proceedings of the National Academy of Sciences, 117(4), 2187-2193.

Thomas, L., & Sharifi, N. (2020). Germline HSD3B1 genetics and prostate cancer outcomes. Urology, 145, 13-21.

Hearn, J. W., Sweeney, C. J., Almassi, N., Reichard, C. A., Reddy, C. A., Li, H., ... & Sharifi, N. (2020). HSD3B1 genotype and clinical outcomes in metastatic castration-sensitive prostate cancer. JAMA oncology, 6(4), e196496-e196496.

Sabharwal, N., & Sharifi, N. (2019). HSD3B1 genotypes conferring adrenal-restrictive and adrenal-permissive phenotypes in prostate cancer and beyond. Endocrinology, 160(9), 2180-2188.

Alyamani, M., Emamekhoo, H., Park, S., Taylor, J., Almassi, N., Upadhyay, S., ... & Sharifi, N. (2018). HSD3B1 (1245A> C) variant regulates dueling abiraterone metabolite effects in prostate cancer. The Journal of Clinical Investigation, 128(8), 3333-3340.

Hettel, D., & Sharifi, N. (2018). HSD3B1 status as a biomarker of androgen deprivation resistance and implications for prostate cancer. Nature Reviews Urology, 15(3), 191-196.

Almassi, N., Reichard, C., Li, J., Russell, C., Perry, J., Ryan, C. J., ... & Sharifi, N. (2018). HSD3B1 and response to a nonsteroidal CYP17A1 inhibitor in castration-resistant prostate cancer. JAMA oncology, 4(4), 554-557.

Hearn, J. W., Xie, W., Nakabayashi, M., Almassi, N., Reichard, C. A., Pomerantz, M., ... & Sharifi, N. (2018). Association of HSD3B1 genotype with response to androgen-deprivation therapy for biochemical recurrence after radiotherapy for localized prostate cancer. JAMA oncology, 4(4), 558-562.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

HSD3B1 Matched Antibody Pair (574) (CAT#: APMAB-574LY)

Antibody Pairs

HSD3B1 Matched Antibody Pair (574) (CAT#: APMAB-574LY)

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Isotype control

For research use only. Not intended for any clinical use.

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