Mouse Anti-KARS Recombinant Antibody (8G12C1) (CBMAB-K0398-LY)

Name: Mouse Anti-KARS Recombinant Antibody (8G12C1)
SKU: CBMAB-K0398-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

8G12C1

Application

IHC, WB

Immunogen

Purified recombinant fragment of KARS(aa90-174) expressed in E. Coli

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Preservative

0.03% sodium azide

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Lysyl-TRNA Synthetase

Introduction

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Entrez Gene ID

3735

UniProt ID

Q15046

Alternative Names

Lysyl-TRNA Synthetase; Lysine TRNA Ligase; EC 6.1.1.6; LysRS; Deafness; Autosomal Recessive 89; Lysine--TRNA Ligase; EC 2.7.7.-; KIAA0070;

Function

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed:9278442, PubMed:18029264, PubMed:18272479).
When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages (PubMed:15851690).
Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity (PubMed:5338216, PubMed:14975237, PubMed:19524539, PubMed:23159739).
(Microbial infection) Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.

Biological Process

Basophil activation involved in immune responseManual Assertion Based On ExperimentIGI:CAFA
Diadenosine tetraphosphate biosynthetic processManual Assertion Based On ExperimentIDA:UniProtKB
ERK1 and ERK2 cascadeManual Assertion Based On ExperimentIGI:CAFA
Lysyl-tRNA aminoacylationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of inflammatory response to antigenic stimulusManual Assertion Based On ExperimentIDA:CAFA
Positive regulation of macrophage activationManual Assertion Based On ExperimentIDA:CAFA
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIGI:CAFA
Response to X-rayIEA:Ensembl
tRNA processing1 PublicationNAS:UniProtKB

PTM

Phosphorylated on a serine residue after mast cell stimulation with immunoglobulin E (IgE).

Scheidecker, S., Bär, S., Stoetzel, C., Geoffroy, V., Lannes, B., Rinaldi, B., ... & Dollfus, H. (2019). Mutations in KARS cause a severe neurological and neurosensory disease with optic neuropathy. Human mutation, 40(10), 1826-1840.

Itoh, M., Dai, H., Horike, S. I., Gonzalez, J., Kitami, Y., Meguro-Horike, M., ... & Kato, Y. (2019). Biallelic KARS pathogenic variants cause an early-onset progressive leukodystrophy. Brain, 142(3), 560-573.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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