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Mouse Anti-NSF Recombinant Antibody (CBXS-0051) (CBMAB-S3052-CQ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBXS-0051
Application
WB, ICC, IHC-P, IHC-Fr, ELISA

Basic Information

Immunogen
N-Ethylmaleimide Sensitive Factor
Specificity
Human
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4, 50% glycerol
Preservative
0.02% sodium azide
Concentration
0.5 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
N-Ethylmaleimide Sensitive Factor, Vesicle Fusing ATPase
Introduction
Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity).
Entrez Gene ID
4905
UniProt ID
P46459
Alternative Names
N-Ethylmaleimide Sensitive Factor, Vesicle Fusing ATPase; N-Ethylmaleimide-Sensitive Factor-Like Protein; N-Ethylmaleimide-Sensitive Fusion Protein; Vesicular-Fusion Protein NSF; NEM-Sensitive Fusion Protein; EC 3.6.4.6;
Function
Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity).
Biological Process
ExocytosisManual Assertion Based On ExperimentTAS:UniProtKB
Golgi to plasma membrane protein transportManual Assertion Based On ExperimentIBA:GO_Central
Intra-Golgi vesicle-mediated transportManual Assertion Based On ExperimentIBA:GO_Central
Intracellular protein transportManual Assertion Based On ExperimentIMP:CACAO
Plasma membrane fusionManual Assertion Based On ExperimentTAS:UniProtKB
Positive regulation of protein catabolic processManual Assertion Based On ExperimentIMP:CACAO
Positive regulation of receptor recyclingManual Assertion Based On ExperimentIDA:UniProtKB
Potassium ion transportIEA:Ensembl
Regulation of exocytosisISS:ParkinsonsUK-UCL
SNARE complex disassemblyISS:ParkinsonsUK-UCL
Vesicle-mediated transportISS:ParkinsonsUK-UCL
Cellular Location
Cytoplasm
Involvement in disease
Developmental and epileptic encephalopathy 96 (DEE96):
A form of epileptic encephalopathy, a heterogeneous group of early-onset epilepsies characterized by refractory seizures, neurodevelopmental impairment, and poor prognosis. Development is normal prior to seizure onset, after which cognitive and motor delays become apparent. DEE96 is an autosomal dominant form characterized by onset of seizures in the first days or weeks of life. Affected infants also have hypotonia with respiratory insufficiency that may result in premature death.
PTM
Phosphorylation at Ser-569 interferes with homohexamerization.
More Infomation

Liu, F., He, R., Xu, X., Zhu, M., Yu, H., & Liu, Y. (2024). Munc18c accelerates SNARE-dependent membrane fusion in the presence of regulatory proteins α-SNAP and NSF. Journal of Biological Chemistry, 105782.

Park, M., Mayer, U., Richter, S., & Jürgens, G. (2023). NSF/αSNAP2-mediated cis-SNARE complex disassembly precedes vesicle fusion in Arabidopsis cytokinesis. Nature Plants, 9(6), 889-897.

Park, M., Mayer, U., & Jürgens, G. (2022). NSF/αSNAP2-mediated cis-SNARE complex disassembly precedes membrane fusion generating the cell plate during Arabidopsis cytokinesis. bioRxiv, 2022-04.

Prikas, E., Paric, E., Asih, P. R., Stefanoska, K., Stefen, H., Fath, T., ... & Ittner, A. (2022). Tau target identification reveals NSF‐dependent effects on AMPA receptor trafficking and memory formation. The EMBO Journal, 41(18), e10242.

Sparks, R. P., Arango, A. S., Starr, M. L., Aboff, Z. L., Hurst, L. R., Rivera-Kohr, D. A., ... & Fratti, R. A. (2019). A small-molecule competitive inhibitor of phosphatidic acid binding by the AAA+ protein NSF/Sec18 blocks the SNARE-priming stage of vacuole fusion. Journal of Biological Chemistry, 294(46), 17168-17185.

Ruete, M. C., Zarelli, V. E. P., Masone, D., de Paola, M., Bustos, D. M., & Tomes, C. N. (2019). A connection between reversible tyrosine phosphorylation and SNARE complex disassembly activity of N-ethylmaleimide-sensitive factor unveiled by the phosphomimetic mutant N-ethylmaleimide-sensitive factor-Y83E. Molecular human reproduction, 25(7), 344-358.

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For research use only. Not intended for any clinical use.

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