Human NSF ELISA Kit (V2LY-0626-LY3824)

Name: Human NSF ELISA Kit
SKU: V2LY-0626-LY3824
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.007 ng/mL

Detection Range

0.015-3 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

82.6 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

N-Ethylmaleimide Sensitive Factor, Vesicle Fusing ATPase

Function

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity).

Biological Process

ExocytosisManual Assertion Based On ExperimentTAS:UniProtKB
Golgi to plasma membrane protein transportManual Assertion Based On ExperimentIBA:GO_Central
Intra-Golgi vesicle-mediated transportManual Assertion Based On ExperimentIBA:GO_Central
Intracellular protein transportManual Assertion Based On ExperimentIMP:CACAO
Plasma membrane fusionManual Assertion Based On ExperimentTAS:UniProtKB
Positive regulation of protein catabolic processManual Assertion Based On ExperimentIMP:CACAO
Positive regulation of receptor recyclingManual Assertion Based On ExperimentIDA:UniProtKB
Potassium ion transportIEA:Ensembl
Regulation of exocytosisISS:ParkinsonsUK-UCL
SNARE complex disassemblyISS:ParkinsonsUK-UCL
Vesicle-mediated transportISS:ParkinsonsUK-UCL

Cellular Location

Cytoplasm

Involvement in disease

Developmental and epileptic encephalopathy 96 (DEE96):
A form of epileptic encephalopathy, a heterogeneous group of early-onset epilepsies characterized by refractory seizures, neurodevelopmental impairment, and poor prognosis. Development is normal prior to seizure onset, after which cognitive and motor delays become apparent. DEE96 is an autosomal dominant form characterized by onset of seizures in the first days or weeks of life. Affected infants also have hypotonia with respiratory insufficiency that may result in premature death.

PTM

Phosphorylation at Ser-569 interferes with homohexamerization.

Liu, F., He, R., Xu, X., Zhu, M., Yu, H., & Liu, Y. (2024). Munc18c accelerates SNARE-dependent membrane fusion in the presence of regulatory proteins α-SNAP and NSF. Journal of Biological Chemistry, 105782.

Park, M., Mayer, U., Richter, S., & Jürgens, G. (2023). NSF/αSNAP2-mediated cis-SNARE complex disassembly precedes vesicle fusion in Arabidopsis cytokinesis. Nature Plants, 9(6), 889-897.

Park, M., Mayer, U., & Jürgens, G. (2022). NSF/αSNAP2-mediated cis-SNARE complex disassembly precedes membrane fusion generating the cell plate during Arabidopsis cytokinesis. bioRxiv, 2022-04.

Prikas, E., Paric, E., Asih, P. R., Stefanoska, K., Stefen, H., Fath, T., ... & Ittner, A. (2022). Tau target identification reveals NSF‐dependent effects on AMPA receptor trafficking and memory formation. The EMBO Journal, 41(18), e10242.

Sparks, R. P., Arango, A. S., Starr, M. L., Aboff, Z. L., Hurst, L. R., Rivera-Kohr, D. A., ... & Fratti, R. A. (2019). A small-molecule competitive inhibitor of phosphatidic acid binding by the AAA+ protein NSF/Sec18 blocks the SNARE-priming stage of vacuole fusion. Journal of Biological Chemistry, 294(46), 17168-17185.

Ruete, M. C., Zarelli, V. E. P., Masone, D., de Paola, M., Bustos, D. M., & Tomes, C. N. (2019). A connection between reversible tyrosine phosphorylation and SNARE complex disassembly activity of N-ethylmaleimide-sensitive factor unveiled by the phosphomimetic mutant N-ethylmaleimide-sensitive factor-Y83E. Molecular human reproduction, 25(7), 344-358.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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