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Mouse Anti-PA2G4 Recombinant Antibody (1D3) (CBMAB-P0620-YC)

Provided herein is a Mouse monoclonal antibody against Human Proliferation-Associated 2G4. The antibody can be used for immunoassay techniques, such as WB.
See all PA2G4 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1D3
Antibody Isotype
IgG1
Application
WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Proliferation-Associated 2G4
Introduction
PA2G4 is an RNA-binding protein that is involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. This protein can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. This protein is also a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases. This protein has been implicated in growth inhibition and the induction of differentiation of human cancer cells. Six pseudogenes, located on chromosomes 3, 6, 9, 18, 20 and X, have been identified.
Entrez Gene ID
5036
UniProt ID
Q9UQ80
Alternative Names
Proliferation-Associated 2G4; Proliferation-Associated 2G4, 38kDa; Proliferation-Associated 2G4, 38kD; Cell Cycle Protein P38-2G4 Homolog; ErbB3-Binding Protein 1; HG4-1;
Function
May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site) (By similarity).
Regulates cell proliferation, differentiation, and survival. Isoform 1 suppresses apoptosis whereas isoform 2 promotes cell differentiation (By similarity).
Biological Process
Negative regulation of apoptotic processISS:UniProtKB
Negative regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:MGI
Positive regulation of cell differentiationISS:UniProtKB
Regulation of translationIEA:UniProtKB-KW
rRNA processingIEA:UniProtKB-KW
Cellular Location
Isoform 1
Cytoplasm
Nucleus, nucleolus
Translocates to the nucleus upon treatment with HRG. Phosphorylation at Ser-361 by PKC/PRKCD regulates its nucleolar localization.
Isoform 2
Cytoplasm
PTM
Phosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent. Phosphorylation at Ser-361 by PKC/PRKCD regulates its nucleolar localization.
In cancer cells, isoform 2 is polyubiquitinated leading to its proteasomal degradation and phosphorylation by PKC/PRKCD enhances polyubiquitination.

Hwang, I., Kim, B. S., Lee, H. Y., Cho, S. W., Lee, S. E., & Ahn, J. Y. (2024). PA2G4/EBP1 ubiquitination by PRKN/PARKIN promotes mitophagy protecting neuron death in cerebral ischemia. Autophagy, 20(2), 365-379.

Massudi, H., Luo, J. S., Holien, J. K., Gadde, S., Krishan, S., Herath, M., ... & Marshall, G. M. (2023). Inhibitors of the Oncogenic PA2G4-MYCN Protein-Protein Interface. Cancers, 15(6), 1822.

Sun, S., Liu, Y., Zhou, M., Wen, J., Xue, L., Han, S., ... & Zhang, B. (2022). PA2G4 promotes the metastasis of hepatocellular carcinoma by stabilizing FYN mRNA in a YTHDF2-dependent manner. Cell & Bioscience, 12(1), 55.

Hou, X., & Tang, W. (2021). Pseudogene PA2G4P4 promotes oncogene PA2G4 expression and nuclear translocation to affect glioblastoma cell viability and apoptosis. Life Sciences, 265, 118793.

Xu, Y., Cai, H., Tu, W., Ding, L., & Luo, R. (2021). Increased PA2G4 expression is an unfavorable factor in nasopharyngeal carcinoma. Applied Immunohistochemistry & Molecular Morphology, 29(7), 513-518.

Stevenson, B. W., Gorman, M. A., Koach, J., Cheung, B. B., Marshall, G. M., Parker, M. W., & Holien, J. K. (2020). A structural view of PA2G4 isoforms with opposing functions in cancer. Journal of Biological Chemistry, 295(47), 16100-16112.

Koach, J., Holien, J. K., Massudi, H., Carter, D. R., Ciampa, O. C., Herath, M., ... & Marshall, G. M. (2019). Drugging MYCN oncogenic signaling through the MYCN-PA2G4 binding interface. Cancer research, 79(21), 5652-5667.

Huang, X., He, M., Huang, S., Lin, R., Zhan, M., Yang, D., ... & Wang, J. (2019). Circular RNA circERBB2 promotes gallbladder cancer progression by regulating PA2G4-dependent rDNA transcription. Molecular Cancer, 18, 1-19.

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For research use only. Not intended for any clinical use.

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