Mouse Anti-PA2G4 Recombinant Antibody (PA2GF5F8) (CBMAB-P0622-YC)

Name: Mouse Anti-PA2G4 Recombinant Antibody (PA2GF5F8)
SKU: CBMAB-P0622-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

PA2GF5F8

Application

Dot

Immunogen

Recombinant human Proliferation-Associated 2G4, 38Kda

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Proliferation-Associated 2G4

Introduction

PA2G4 is an RNA-binding protein that is involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. This protein can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. This protein is also a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases. This protein has been implicated in growth inhibition and the induction of differentiation of human cancer cells. Six pseudogenes, located on chromosomes 3, 6, 9, 18, 20 and X, have been identified.

Entrez Gene ID

5036

UniProt ID

Q9UQ80

Alternative Names

Proliferation-Associated 2G4; Proliferation-Associated 2G4, 38kDa; Proliferation-Associated 2G4, 38kD; Cell Cycle Protein P38-2G4 Homolog; ErbB3-Binding Protein 1; HG4-1;

Function

May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site) (By similarity).
Regulates cell proliferation, differentiation, and survival. Isoform 1 suppresses apoptosis whereas isoform 2 promotes cell differentiation (By similarity).

Biological Process

Negative regulation of apoptotic processISS:UniProtKB
Negative regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:MGI
Positive regulation of cell differentiationISS:UniProtKB
Regulation of translationIEA:UniProtKB-KW
rRNA processingIEA:UniProtKB-KW

Cellular Location

Isoform 1
Cytoplasm
Nucleus, nucleolus
Translocates to the nucleus upon treatment with HRG. Phosphorylation at Ser-361 by PKC/PRKCD regulates its nucleolar localization.
Isoform 2
Cytoplasm

PTM

Phosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent. Phosphorylation at Ser-361 by PKC/PRKCD regulates its nucleolar localization.
In cancer cells, isoform 2 is polyubiquitinated leading to its proteasomal degradation and phosphorylation by PKC/PRKCD enhances polyubiquitination.

Hwang, I., Kim, B. S., Lee, H. Y., Cho, S. W., Lee, S. E., & Ahn, J. Y. (2024). PA2G4/EBP1 ubiquitination by PRKN/PARKIN promotes mitophagy protecting neuron death in cerebral ischemia. Autophagy, 20(2), 365-379.

Massudi, H., Luo, J. S., Holien, J. K., Gadde, S., Krishan, S., Herath, M., ... & Marshall, G. M. (2023). Inhibitors of the Oncogenic PA2G4-MYCN Protein-Protein Interface. Cancers, 15(6), 1822.

Sun, S., Liu, Y., Zhou, M., Wen, J., Xue, L., Han, S., ... & Zhang, B. (2022). PA2G4 promotes the metastasis of hepatocellular carcinoma by stabilizing FYN mRNA in a YTHDF2-dependent manner. Cell & Bioscience, 12(1), 55.

Hou, X., & Tang, W. (2021). Pseudogene PA2G4P4 promotes oncogene PA2G4 expression and nuclear translocation to affect glioblastoma cell viability and apoptosis. Life Sciences, 265, 118793.

Xu, Y., Cai, H., Tu, W., Ding, L., & Luo, R. (2021). Increased PA2G4 expression is an unfavorable factor in nasopharyngeal carcinoma. Applied Immunohistochemistry & Molecular Morphology, 29(7), 513-518.

Stevenson, B. W., Gorman, M. A., Koach, J., Cheung, B. B., Marshall, G. M., Parker, M. W., & Holien, J. K. (2020). A structural view of PA2G4 isoforms with opposing functions in cancer. Journal of Biological Chemistry, 295(47), 16100-16112.

Koach, J., Holien, J. K., Massudi, H., Carter, D. R., Ciampa, O. C., Herath, M., ... & Marshall, G. M. (2019). Drugging MYCN oncogenic signaling through the MYCN-PA2G4 binding interface. Cancer research, 79(21), 5652-5667.

Huang, X., He, M., Huang, S., Lin, R., Zhan, M., Yang, D., ... & Wang, J. (2019). Circular RNA circERBB2 promotes gallbladder cancer progression by regulating PA2G4-dependent rDNA transcription. Molecular Cancer, 18, 1-19.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

PA2G4 Matched Antibody Pair (839) (CAT#: APMAB-839LY)

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PA2G4 Matched Antibody Pair (839) (CAT#: APMAB-839LY)

Isotype control

For research use only. Not intended for any clinical use.

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