Human Recombinant EBP1 protein, His Tag (V2LY-0526-LY3671)

Name: Human Recombinant EBP1 protein, His Tag
SKU: V2LY-0526-LY3671
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant EBP1 protein, His Tag consist of Amino Acid: 2-394 and predicts a molecular mass of 45.2 kDa.

Molecule Mass

45.2 kDa

Sequence

Amino Acid: 2-394

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>92% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Proliferation-Associated 2G4

Function

May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site) (By similarity).
Regulates cell proliferation, differentiation, and survival. Isoform 1 suppresses apoptosis whereas isoform 2 promotes cell differentiation (By similarity).

Biological Process

Negative regulation of apoptotic processISS:UniProtKB
Negative regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:MGI
Positive regulation of cell differentiationISS:UniProtKB
Regulation of translationIEA:UniProtKB-KW
rRNA processingIEA:UniProtKB-KW

Cellular Location

Isoform 1
Cytoplasm
Nucleus, nucleolus
Translocates to the nucleus upon treatment with HRG. Phosphorylation at Ser-361 by PKC/PRKCD regulates its nucleolar localization.
Isoform 2
Cytoplasm

PTM

Phosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent. Phosphorylation at Ser-361 by PKC/PRKCD regulates its nucleolar localization.
In cancer cells, isoform 2 is polyubiquitinated leading to its proteasomal degradation and phosphorylation by PKC/PRKCD enhances polyubiquitination.

Hwang, I., Kim, B. S., Lee, H. Y., Cho, S. W., Lee, S. E., & Ahn, J. Y. (2024). PA2G4/EBP1 ubiquitination by PRKN/PARKIN promotes mitophagy protecting neuron death in cerebral ischemia. Autophagy, 20(2), 365-379.

Massudi, H., Luo, J. S., Holien, J. K., Gadde, S., Krishan, S., Herath, M., ... & Marshall, G. M. (2023). Inhibitors of the Oncogenic PA2G4-MYCN Protein-Protein Interface. Cancers, 15(6), 1822.

Sun, S., Liu, Y., Zhou, M., Wen, J., Xue, L., Han, S., ... & Zhang, B. (2022). PA2G4 promotes the metastasis of hepatocellular carcinoma by stabilizing FYN mRNA in a YTHDF2-dependent manner. Cell & Bioscience, 12(1), 55.

Hou, X., & Tang, W. (2021). Pseudogene PA2G4P4 promotes oncogene PA2G4 expression and nuclear translocation to affect glioblastoma cell viability and apoptosis. Life Sciences, 265, 118793.

Xu, Y., Cai, H., Tu, W., Ding, L., & Luo, R. (2021). Increased PA2G4 expression is an unfavorable factor in nasopharyngeal carcinoma. Applied Immunohistochemistry & Molecular Morphology, 29(7), 513-518.

Stevenson, B. W., Gorman, M. A., Koach, J., Cheung, B. B., Marshall, G. M., Parker, M. W., & Holien, J. K. (2020). A structural view of PA2G4 isoforms with opposing functions in cancer. Journal of Biological Chemistry, 295(47), 16100-16112.

Koach, J., Holien, J. K., Massudi, H., Carter, D. R., Ciampa, O. C., Herath, M., ... & Marshall, G. M. (2019). Drugging MYCN oncogenic signaling through the MYCN-PA2G4 binding interface. Cancer research, 79(21), 5652-5667.

Huang, X., He, M., Huang, S., Lin, R., Zhan, M., Yang, D., ... & Wang, J. (2019). Circular RNA circERBB2 promotes gallbladder cancer progression by regulating PA2G4-dependent rDNA transcription. Molecular Cancer, 18, 1-19.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

PA2G4 Matched Antibody Pair (839) (CAT#: APMAB-839LY)

Antibody Pairs

PA2G4 Matched Antibody Pair (839) (CAT#: APMAB-839LY)

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Isotype control

For research use only. Not intended for any clinical use.

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