Human PA2G4 ELISA Kit (V2LY-0626-LY3141)

Name: Human PA2G4 ELISA Kit
SKU: V2LY-0626-LY3141
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0088 ng/mL

Detection Range

0.02-3.8 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

43.8 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Proliferation-Associated 2G4

Function

May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site) (By similarity).
Regulates cell proliferation, differentiation, and survival. Isoform 1 suppresses apoptosis whereas isoform 2 promotes cell differentiation (By similarity).

Biological Process

Negative regulation of apoptotic processISS:UniProtKB
Negative regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:MGI
Positive regulation of cell differentiationISS:UniProtKB
Regulation of translationIEA:UniProtKB-KW
rRNA processingIEA:UniProtKB-KW

Cellular Location

Isoform 1
Cytoplasm
Nucleus, nucleolus
Translocates to the nucleus upon treatment with HRG. Phosphorylation at Ser-361 by PKC/PRKCD regulates its nucleolar localization.
Isoform 2
Cytoplasm

PTM

Phosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent. Phosphorylation at Ser-361 by PKC/PRKCD regulates its nucleolar localization.
In cancer cells, isoform 2 is polyubiquitinated leading to its proteasomal degradation and phosphorylation by PKC/PRKCD enhances polyubiquitination.

Hwang, I., Kim, B. S., Lee, H. Y., Cho, S. W., Lee, S. E., & Ahn, J. Y. (2024). PA2G4/EBP1 ubiquitination by PRKN/PARKIN promotes mitophagy protecting neuron death in cerebral ischemia. Autophagy, 20(2), 365-379.

Massudi, H., Luo, J. S., Holien, J. K., Gadde, S., Krishan, S., Herath, M., ... & Marshall, G. M. (2023). Inhibitors of the Oncogenic PA2G4-MYCN Protein-Protein Interface. Cancers, 15(6), 1822.

Sun, S., Liu, Y., Zhou, M., Wen, J., Xue, L., Han, S., ... & Zhang, B. (2022). PA2G4 promotes the metastasis of hepatocellular carcinoma by stabilizing FYN mRNA in a YTHDF2-dependent manner. Cell & Bioscience, 12(1), 55.

Hou, X., & Tang, W. (2021). Pseudogene PA2G4P4 promotes oncogene PA2G4 expression and nuclear translocation to affect glioblastoma cell viability and apoptosis. Life Sciences, 265, 118793.

Xu, Y., Cai, H., Tu, W., Ding, L., & Luo, R. (2021). Increased PA2G4 expression is an unfavorable factor in nasopharyngeal carcinoma. Applied Immunohistochemistry & Molecular Morphology, 29(7), 513-518.

Stevenson, B. W., Gorman, M. A., Koach, J., Cheung, B. B., Marshall, G. M., Parker, M. W., & Holien, J. K. (2020). A structural view of PA2G4 isoforms with opposing functions in cancer. Journal of Biological Chemistry, 295(47), 16100-16112.

Koach, J., Holien, J. K., Massudi, H., Carter, D. R., Ciampa, O. C., Herath, M., ... & Marshall, G. M. (2019). Drugging MYCN oncogenic signaling through the MYCN-PA2G4 binding interface. Cancer research, 79(21), 5652-5667.

Huang, X., He, M., Huang, S., Lin, R., Zhan, M., Yang, D., ... & Wang, J. (2019). Circular RNA circERBB2 promotes gallbladder cancer progression by regulating PA2G4-dependent rDNA transcription. Molecular Cancer, 18, 1-19.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

PA2G4 Matched Antibody Pair (839) (CAT#: APMAB-839LY)

Human Recombinant EBP1 protein, His Tag (CAT#: V2LY-0526-LY3671)

Antibody Pairs

PA2G4 Matched Antibody Pair (839) (CAT#: APMAB-839LY)

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Mouse Anti-PA2G4 Recombinant Antibody (2A5) (CAT#: CBMAB-P0621-YC)

Mouse Anti-PA2G4 (AA 1-110) Recombinant Antibody (CBFYE-1573) (CAT#: CBMAB-E2227-FY)

Human Recombinant EBP1 protein, His Tag (CAT#: V2LY-0526-LY3671)

PA2G4 Matched Antibody Pair (839) (CAT#: APMAB-839LY)

Mouse Anti-PA2G4 Recombinant Antibody (PA2GF5F8) (CAT#: CBMAB-P0622-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

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