Rabbit Anti-POSTN Recombinant Antibody (
14F2) (V2LY-0725-LY944)

Name: Rabbit Anti-POSTN Recombinant Antibody (14F2)
SKU: V2LY-0725-LY944
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-POSTN Recombinant Antibody (14F2) (V2LY-0725-LY944) in IHC

IHC image of V2LY-0725-LY944 diluted at 1:50 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.31% DAB.

Rabbit Anti-POSTN Recombinant Antibody (14F2) (V2LY-0725-LY944) in FC

Overlay Peak curve showing 293T cells stained with V2LY-0725-LY944 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

14F2

Application

ELISA, IHC, FC

Immunogen

A synthesized peptide derived from Human POSTN.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IHC	1:50-1:200
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Periostin

Entrez Gene ID

10631

UniProt ID

Q15063

Function

Induces cell attachment and spreading and plays a role in cell adhesion (PubMed:12235007).
Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity).

Biological Process

Cell adhesionManual Assertion Based On ExperimentIDA:UniProtKB
Cellular response to vitamin KManual Assertion Based On ExperimentIDA:UniProtKB
Extracellular matrix organizationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of Notch signaling pathwayIEA:Ensembl
Tissue developmentIEA:Ensembl

Cellular Location

Golgi apparatus
Secreted
Secreted, extracellular space, extracellular matrix
Colocalizes with BMP1 in the Golgi.

PTM

Gamma-carboxylation is controversial. Gamma-carboxyglutamated; gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation; this may be required for calcium binding (PubMed:18450759).
According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues (PubMed:26273833).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-POSTN Recombinant Antibody (Stiny-1) (CAT#: CBMAB-P2422-YC)

Mouse Anti-POSTN Recombinant Antibody (2D10) (CAT#: CBMAB-P2417-YC)

Mouse Anti-POSTN Recombinant Antibody (F-10) (CAT#: CBMAB-P2421-YC)

Rat Anti-POSTN Recombinant Antibody (CBXO-0280) (CAT#: CBMAB-O0645-CQ)

Mouse Postn ELISA Kit (CAT#: V2LY-0626-LY2854)

Mouse Anti-POSTN (AA 1-230) Recombinant Antibody (CBXO-0536) (CAT#: CBMAB-O0643-CQ)

Human Recombinant POSTN protein, His Tag (CAT#: V2LY-0526-LY6110)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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