Mouse Anti-UBA2 Recombinant Antibody (CBFYU-023) (CBMAB-U0023-FY)

Name: Mouse Anti-UBA2 Recombinant Antibody (CBFYU-023)
SKU: CBMAB-U0023-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBFYU-023

Application

WB, IHC, IF

Immunogen

Full length human recombinant protein of human UBA2 (NP_005490) produced in HEK293T cell

Specificity

Human, Dog, Rat, Monkey

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS(pH 7.3) containing 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Concentration

0.41 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ubiquitin like modifier activating enzyme 2

Introduction

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Entrez Gene ID

Human	10054
Rat	308508
Monkey	705808
Dog	476490

UniProt ID

Human	Q9UBT2
Rat	F1LS72
Monkey	F6TLE0
Dog	A0A8C0M2R3

Alternative Names

ARX; SAE2; HRIHFB2115

Function

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Biological Process

Biological Process positive regulation of protein sumoylation Source:UniProtKB1 Publication
Biological Process protein modification by small protein conjugation Source:GO_Central1 Publication
Biological Process protein sumoylation Source:UniProtKB1 Publication

Cellular Location

Cytoplasm
Nucleus
Shuttles between the cytoplasm and the nucleus, sumoylation is required either for nuclear translocation or nuclear retention.

PTM

Sumoylated with SUMO1 and SUMO2/3 and by UBC9. Sumoylation at Lys-236 inhibits enzymatic activity. Sumoylation at the C-terminal lysine cluster plays an essential role in nuclear trafficking.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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