Cattle IFI30 ELISA Kit (V2LY-0626-LY726)

Name: Cattle IFI30 ELISA Kit
SKU: V2LY-0626-LY726
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.01 ng/mL

Detection Range

0.05-15 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Cattle

Assay Type

Sandwich

Reactivity

Cattle

Assay Time

1.5 h

Molecule Mass

27.5 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Ifi30, Lysosomal Thiol Reductase

Function

Lysosomal thiol reductase that can reduce protein disulfide bonds. May facilitate the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing. Facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds (By similarity).

Facilitates also MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation (By similarity).

Biological Process

Antigen processing and presentation of exogenous peptide antigen via MHC class I Source: UniProtKB
Antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
Negative regulation of fibroblast proliferation Source: Ensembl
Protein stabilization Source: Ensembl

Cellular Location

Lysosome; Secreted

PTM

N-glycosylated. Sugar chains contain mannose-6-phosphate.
Synthesized as a 35 kDa precursor which is then processed into the mature 30 kDa form via cleavage of N-terminal and C-terminal propeptides. Processing of the precursor is mediated by multiple lysosomal proteases.

Chen, Y., Xu, H., Yu, P., Wang, Q., Li, S., Ji, F., ... & Lan, Q. (2023). Interferon‐γ inducible protein 30 promotes the epithelial–mesenchymal transition‐like phenotype and chemoresistance by activating EGFR/AKT/GSK3β/β‐catenin pathway in glioma. CNS Neuroscience & Therapeutics.

Wang, X., Ge, X., Qin, Y., Liu, D., & Chen, C. (2022). Ifi30 Is Required for Sprouting Angiogenesis During Caudal Vein Plexus Formation in Zebrafish. Frontiers in Physiology, 13, 919579.

Fan, Y., Wang, X., & Li, Y. (2021). IFI30 expression predicts patient prognosis in breast cancer and dictates breast cancer cells proliferation via regulating autophagy. International Journal of Medical Sciences, 18(14), 3342.

Jiang, W., Zheng, F., Yao, T., Gong, F., Zheng, W., & Yao, N. (2021). IFI30 as a prognostic biomarker and correlation with immune infiltrates in glioma. Annals of Translational Medicine, 9(22).

Zhu, C., Chen, X., Guan, G., Zou, C., Guo, Q., Cheng, P., ... & Wu, A. (2020). IFI30 is a novel immune-related target with predicting value of prognosis and treatment response in glioblastoma. OncoTargets and therapy, 1129-1143.

Guo, J., Zhou, M., Liu, X., Pan, Y., Yang, R., Zhao, Z., & Sun, B. (2018). Porcine IFI30 inhibits PRRSV proliferation and host cell apoptosis in vitro. Gene, 649, 93-98.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant IFI30 protein, His Tag (CAT#: V2LY-0526-LY4643)

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Human Recombinant IFI30 protein, His Tag (CAT#: V2LY-0526-LY4643)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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