Human Recombinant IFI30 protein, His Tag (V2LY-0526-LY4643)

Name: Human Recombinant IFI30 protein, His Tag
SKU: V2LY-0526-LY4643
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant IFI30 protein, His Tag consist of Amino Acid: 1-232 and predicts a molecular mass of 24.7 kDa.

Molecule Mass

24.7 kDa

Sequence

Amino Acid: 1-232

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>96% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Sodium phosphate, NaCl, Imidazole, PMSF, DTT, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Ifi30, Lysosomal Thiol Reductase

Function

Lysosomal thiol reductase that can reduce protein disulfide bonds. May facilitate the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing. Facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds (By similarity).

Facilitates also MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation (By similarity).

Biological Process

Antigen processing and presentation of exogenous peptide antigen via MHC class I Source: UniProtKB
Antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
Negative regulation of fibroblast proliferation Source: Ensembl
Protein stabilization Source: Ensembl

Cellular Location

Lysosome; Secreted

PTM

N-glycosylated. Sugar chains contain mannose-6-phosphate.
Synthesized as a 35 kDa precursor which is then processed into the mature 30 kDa form via cleavage of N-terminal and C-terminal propeptides. Processing of the precursor is mediated by multiple lysosomal proteases.

Chen, Y., Xu, H., Yu, P., Wang, Q., Li, S., Ji, F., ... & Lan, Q. (2023). Interferon‐γ inducible protein 30 promotes the epithelial–mesenchymal transition‐like phenotype and chemoresistance by activating EGFR/AKT/GSK3β/β‐catenin pathway in glioma. CNS Neuroscience & Therapeutics.

Wang, X., Ge, X., Qin, Y., Liu, D., & Chen, C. (2022). Ifi30 Is Required for Sprouting Angiogenesis During Caudal Vein Plexus Formation in Zebrafish. Frontiers in Physiology, 13, 919579.

Fan, Y., Wang, X., & Li, Y. (2021). IFI30 expression predicts patient prognosis in breast cancer and dictates breast cancer cells proliferation via regulating autophagy. International Journal of Medical Sciences, 18(14), 3342.

Jiang, W., Zheng, F., Yao, T., Gong, F., Zheng, W., & Yao, N. (2021). IFI30 as a prognostic biomarker and correlation with immune infiltrates in glioma. Annals of Translational Medicine, 9(22).

Zhu, C., Chen, X., Guan, G., Zou, C., Guo, Q., Cheng, P., ... & Wu, A. (2020). IFI30 is a novel immune-related target with predicting value of prognosis and treatment response in glioblastoma. OncoTargets and therapy, 1129-1143.

Guo, J., Zhou, M., Liu, X., Pan, Y., Yang, R., Zhao, Z., & Sun, B. (2018). Porcine IFI30 inhibits PRRSV proliferation and host cell apoptosis in vitro. Gene, 649, 93-98.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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