Cattle Recombinant PAG1 protein (V2LY-0526-LY1188)

Name: Cattle Recombinant PAG1 protein
SKU: V2LY-0526-LY1188
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Cattle

Protein Construction

This product is Cattle Recombinant PAG1 protein consist of Please contact us for more information. and predicts a molecular mass of 38.1 kDa.

Molecule Mass

38.1 kDa

Sequence

Please contact us for more information.

Species

Cattle

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Phosphoprotein Membrane Anchor With Glycosphingolipid Microdomains 1

Function

Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.

Biological Process

Adaptive immune responseIEA:UniProtKB-KW
Intracellular signal transductionManual Assertion Based On ExperimentIDA:HGNC-UCL
Negative regulation of T cell activationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of T cell activationManual Assertion Based On ExperimentIDA:ProtInc
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Cell membrane

Topology

Extracellular: 1-16
Helical: 17-37
Cytoplasmic: 38-432

PTM

Palmitoylated.
Phosphorylated by FYN on Tyr-317 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation. Phosphorylated by LYN.

Sun, Y., Yang, X., Guan, S., Ma, T., Jiang, Z., Gao, M., ... & Cong, B. (2023). The role of phosphoprotein associated with glycosphingolipid-enriched microdomains 1 (PAG1) in regulating the progression of oral squamous cell carcinoma. Archives of Oral Biology, 156, 105810.

Abd el-Aziz, A., El-Desouky, M. A., Shafei, A., Elnakib, M., & Abdelmoniem, A. M. (2021). Influence of pentoxifylline on gene expression of PAG1/miR-1206/SNHG14 in ischemic heart disease. Biochemistry and Biophysics Reports, 25, 100911.

Foltz, L., Palacios-Moreno, J., Mayfield, M., Kinch, S., Dillon, J., Syrenne, J., ... & Grimes, M. (2020). PAG1 directs SRC-family kinase intracellular localization to mediate receptor tyrosine kinase-induced differentiation. Molecular Biology of the Cell, 31(20), 2269-2282.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Pig PAG1 ELISA Kit (CAT#: V2LY-0626-LY1022)

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Pig PAG1 ELISA Kit (CAT#: V2LY-0626-LY1022)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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