Human ECE2 ELISA Kit (V2LY-0626-LY4544)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0097 ng/mL
Detection Range
0.02-6 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
91.2 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Endothelin Converting Enzyme 2
Research Area
Converts big endothelin-1 to endothelin-1. Also involved in the processing of various neuroendocrine peptides, including neurotensin, angiotensin I, substance P, proenkephalin-derived peptides, and prodynorphin-derived peptides. May play a role in amyloid-beta processing (By similarity).
Biological Process
Cell-cell signaling Source: UniProtKB
G protein-coupled receptor signaling pathway Source: Reactome
Methylation Source: UniProtKB-KW
Peptide hormone processing Source: UniProtKB
Protein processing Source: GO_Central
Cellular Location
Golgi apparatus membrane; Secretory vesicle membrane
Topology
Cytoplasmic: 1-106
Helical: 107-127
Lumenal: 128-811

Turner, A. J., & Nalivaeva, N. N. (2022). Angiotensin-converting enzyme 2 (ACE2): Two decades of revelations and re-evaluation. Peptides, 170766.

Buchsbaum, I. Y., Kielkowski, P., Giorgio, G., O'Neill, A. C., Di Giaimo, R., Kyrousi, C., ... & Cappello, S. (2020). ECE 2 regulates neurogenesis and neuronal migration during human cortical development. EMBO reports, 21(5), e48204.

Liao, X., Cai, F., Sun, Z., Zhang, Y., Wang, J., Jiao, B., ... & Song, W. (2020). Identification of Alzheimer’s disease–associated rare coding variants in the ECE2 gene. JCI insight, 5(4).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant ECE2 protein, hFc Tag (CAT#: V2LY-0526-LY3678)

Human Recombinant ECE2 protein, His Tag (CAT#: V2LY-0526-LY3679)

Related Products

Human Recombinant ECE2 protein, His Tag (CAT#: V2LY-0526-LY3679)

Human Recombinant ECE2 protein, hFc Tag (CAT#: V2LY-0526-LY3678)

Rat Anti-ECE2 Recombinant Antibody (CBFYE-0403) (CAT#: CBMAB-E0715-FY)

Rat Anti-ECE2 Recombinant Antibody (CBFYE-0403) (CAT#: V2LY-0425-LY1333)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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