Human KIR3DL1 ELISA Kit (V2LY-0626-LY4)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.01 ng/mL
Detection Range
0.02-3.8 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
49.1 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Killer Cell Immunoglobulin Like Receptor, Three Ig Domains And Long Cytoplasmic Tail 1
Function
Receptor on natural killer (NK) cells for HLA Bw4 allele. Inhibits the activity of NK cells thus preventing cell lysis.
Biological Process
Immune response2 PublicationsNAS:UniProtKB
Natural killer cell mediated cytotoxicityManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Cell membrane
Topology
Extracellular: 22-340
Helical: 341-360
Cytoplasmic: 361-444

Muriuki, B. M., Forconi, C. S., Kirwa, E. K., Maina, T. K., Ariera, B. O., Bailey, J. A., ... & Ong’echa, J. M. (2023). Evaluation of KIR3DL1/KIR3DS1 allelic polymorphisms in Kenyan children with endemic Burkitt lymphoma. Plos one, 18(8), e0275046.

Harrison, G. F., Leaton, L. A., Harrison, E. A., Kichula, K. M., Viken, M. K., Shortt, J., ... & Norman, P. J. (2022). Allele imputation for the killer cell immunoglobulin-like receptor KIR3DL1/S1. PLoS Computational Biology, 18(2), e1009059.

Izumi, K., Shindo, T., Ngo, H. T., Nakayama-Hosoya, K., Akahane, K., Tamai, M., ... & Takaori-Kondo, A. (2021). KIR3DL1 Allotype-Dependent Modulation of NK Cell Immunity against Chronic Myeloid Leukemia. ImmunoHorizons, 5(8), 687-702.

Schetelig, J., Baldauf, H., Heidenreich, F., Massalski, C., Frank, S., Sauter, J., ... & Bornhäuser, M. (2020). External validation of models for KIR2DS1/KIR3DL1-informed selection of hematopoietic cell donors fails. Blood, The Journal of the American Society of Hematology, 135(16), 1386-1395.

Geng, J., & Raghavan, M. (2019). CD8αα homodimers function as a coreceptor for KIR3DL1. Proceedings of the National Academy of Sciences, 116(36), 17951-17956.

Lisovsky, I., Kant, S., Tremblay-McLean, A., Isitman, G., Kiani, Z., Dupuy, F. P., ... & Bernard, N. F. (2019). Differential contribution of education through KIR2DL1, KIR2DL3, and KIR3DL1 to antibody-dependent (AD) NK cell activation and ADCC. Journal of leukocyte biology, 105(3), 551-563.

Castaño-Núñez, Á., Montes-Cano, M. A., García-Lozano, J. R., Ortego-Centeno, N., García-Hernández, F. J., Espinosa, G., ... & González-Escribano, M. F. (2019). Association of functional polymorphisms of KIR3DL1/DS1 with Behcet's disease. Frontiers in immunology, 10, 2755.

Petrushkin, H., Norman, P. J., Lougee, E., Parham, P., Wallace, G. R., Stanford, M. R., & Fortune, F. (2019). KIR3DL1/S1 allotypes contribute differentially to the development of Behçet disease. The Journal of Immunology, 203(6), 1629-1635.

Pugh, J., Nemat-Gorgani, N., Djaoud, Z., Guethlein, L. A., Norman, P. J., & Parham, P. (2019). In vitro education of human natural killer cells by KIR3DL1. Life Science Alliance, 2(6).

Zhang, X., Lu, X., Moog, C., Yuan, L., Liu, Z., Li, Z., ... & Su, B. (2018). KIR3DL1-negative CD8 T cells and KIR3DL1-negative natural killer cells contribute to the advantageous control of early human immunodeficiency virus type 1 infection in HLA-B Bw4 homozygous individuals. Frontiers in Immunology, 9, 1855.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant KIR3DL1 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY5173)

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For research use only. Not intended for any clinical use.

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