Human NUDT1 ELISA Kit (V2LY-0626-LY4030)

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Tested Data
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.016 ng/mL
Detection Range
0.031-2 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
22.5 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Nudix Hydrolase 1
Function
Oxidized purine nucleoside triphosphate hydrolase which is a prominent sanitizer of the oxidized nucleotide pool (PubMed:8226881, PubMed:7713500, PubMed:10608900, PubMed:12857738, PubMed:22556419, PubMed:26238318, PubMed:24695224, PubMed:24695225, PubMed:28679043).
Catalyzes the hydrolysis of 2-oxo-dATP (2-hydroxy-dATP) into 2-oxo-dAMP (PubMed:10373420).
Has also a significant hydrolase activity toward 2-oxo-ATP, 8-oxo-dGTP and 8-oxo-dATP (PubMed:10373420, PubMed:11139615).
Through the hydrolysis of oxidized purine nucleoside triphosphates, prevents their incorporation into DNA and the subsequent transversions A:T to C:G and G:C to T:A (PubMed:8226881, PubMed:10373420, PubMed:10608900, PubMed:11756418, PubMed:12857738, PubMed:16607562, PubMed:24695224, PubMed:24695225, PubMed:26999531, PubMed:28035004).
Also catalyzes the hydrolysis of methylated purine nucleoside triphosphate preventing their integration into DNA (PubMed:30304478, PubMed:32144205).
Through this antimutagenic activity protects cells from oxidative stress (PubMed:8226881, PubMed:7713500, PubMed:10608900, PubMed:12857738, PubMed:24695224, PubMed:24695225, PubMed:30304478, PubMed:32144205).
Biological Process
AgingIEA:Ensembl
DNA protectionManual Assertion Based On ExperimentIDA:UniProtKB
DNA repair1 PublicationIC:UniProtKB
Male gonad developmentIEA:Ensembl
Purine nucleoside catabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Response to cadmium ionIEA:Ensembl
Response to oxidative stressManual Assertion Based On ExperimentTAS:ProtInc
Cellular Location
Isoform p18
Cytoplasm, cytosol
Mitochondrion matrix
Nucleus
Mostly present in cytosol (PubMed:7782328).
A minor proportion is mitochondrial (PubMed:7782328).
A very small amount of the protein is associated with nuclei (PubMed:7782328).
Isoform p26
Mitochondrion matrix
PTM
The N-terminus is blocked.

Lau, H. C., Yuan, X., Huang, H., Zhang, M., Hsueh, C. Y., & Gong, H. (2023). Fusobacterium nucleatum facilitates proliferation and autophagy by activating miR-361-3p/NUDT1 axis through oxidative stress in hypopharyngeal squamous cell carcinoma. BMC cancer, 23(1), 990.

Lin, Y., Zhang, F., Jin, Y., Zhong, Q., Tan, W., Liu, J., & Wu, Z. (2022). NUDT1 could be a prognostic biomarker and correlated with immune infiltration in clear cell renal cell carcinoma. Applied bionics and biomechanics, 2022.

Huang, B., Lyu, Z., Qian, Q., Chen, Y., Zhang, J., Li, B., ... & Ma, X. (2022). NUDT1 promotes the accumulation and longevity of CD103+ TRM cells in primary biliary cholangitis. Journal of hepatology, 77(5), 1311-1324.

Shi, J., Xiong, Z., Wang, K., Yuan, C., Huang, Y., Xiao, W., ... & Zhang, X. (2021). HIF2α promotes tumour growth in clear cell renal cell carcinoma by increasing the expression of NUDT1 to reduce oxidative stress. Clinical and translational medicine, 11(11), e592.

Bialkowski, K., & Szpila, A. (2021). Specific 8-oxo-dGTPase activity of MTH1 (NUDT1) protein as a quantitative marker and prognostic factor in human colorectal cancer. Free Radical Biology and Medicine, 176, 257-264.

Vitry, G., Paulin, R., Grobs, Y., Lampron, M. C., Shimauchi, T., Lemay, S. E., ... & Bonnet, S. (2021). Oxidized DNA precursors cleanup by NUDT1 contributes to vascular remodeling in pulmonary arterial hypertension. American Journal of Respiratory and Critical Care Medicine, 203(5), 614-627.

Agarwal, S., & de Jesus Perez, V. A. (2021). In defense of the Nucleus: NUDT1 and oxidative DNA damage in pulmonary arterial Hypertension. American Journal of Respiratory and Critical Care Medicine, 203(5), 541-542.

Shen, Y., Zhang, L., Piao, S., Li, L., Li, J., Xia, Y., ... & Saiyin, W. (2020). NUDT1: A potential independent predictor for the prognosis of patients with oral squamous cell carcinoma. Journal of Oral Pathology & Medicine, 49(3), 210-218.

Bialkowski, K., & Kasprzak, K. S. (2020). A profile of 8-oxo-dGTPase activities in the NCI-60 human cancer panel: meta-analytic insight into the regulation and role of MTH1 (NUDT1) gene expression in carcinogenesis. Free Radical Biology and Medicine, 148, 1-21.

Tahara, Y. K., Kietrys, A. M., Hebenbrock, M., Lee, Y., Wilson, D. L., & Kool, E. T. (2019). Dual inhibitors of 8-oxoguanine surveillance by OGG1 and NUDT1. ACS chemical biology, 14(12), 2606-2615.

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For research use only. Not intended for any clinical use.

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