Human Recombinant MTH1 protein, His Tag (V2LY-0526-LY5650)

Name: Human Recombinant MTH1 protein, His Tag
SKU: V2LY-0526-LY5650
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant MTH1 protein, His Tag consist of Amino Acid: 1-156 and predicts a molecular mass of 20.2 kDa.

Molecule Mass

20.2 kDa

Sequence

Amino Acid: 1-156

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Sodium phosphate, NaCl, Imidazole, PMSF, DTT, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Nudix Hydrolase 1

Function

Oxidized purine nucleoside triphosphate hydrolase which is a prominent sanitizer of the oxidized nucleotide pool (PubMed:8226881, PubMed:7713500, PubMed:10608900, PubMed:12857738, PubMed:22556419, PubMed:26238318, PubMed:24695224, PubMed:24695225, PubMed:28679043).
Catalyzes the hydrolysis of 2-oxo-dATP (2-hydroxy-dATP) into 2-oxo-dAMP (PubMed:10373420).
Has also a significant hydrolase activity toward 2-oxo-ATP, 8-oxo-dGTP and 8-oxo-dATP (PubMed:10373420, PubMed:11139615).
Through the hydrolysis of oxidized purine nucleoside triphosphates, prevents their incorporation into DNA and the subsequent transversions A:T to C:G and G:C to T:A (PubMed:8226881, PubMed:10373420, PubMed:10608900, PubMed:11756418, PubMed:12857738, PubMed:16607562, PubMed:24695224, PubMed:24695225, PubMed:26999531, PubMed:28035004).
Also catalyzes the hydrolysis of methylated purine nucleoside triphosphate preventing their integration into DNA (PubMed:30304478, PubMed:32144205).
Through this antimutagenic activity protects cells from oxidative stress (PubMed:8226881, PubMed:7713500, PubMed:10608900, PubMed:12857738, PubMed:24695224, PubMed:24695225, PubMed:30304478, PubMed:32144205).

Biological Process

AgingIEA:Ensembl
DNA protectionManual Assertion Based On ExperimentIDA:UniProtKB
DNA repair1 PublicationIC:UniProtKB
Male gonad developmentIEA:Ensembl
Purine nucleoside catabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Response to cadmium ionIEA:Ensembl
Response to oxidative stressManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Isoform p18
Cytoplasm, cytosol
Mitochondrion matrix
Nucleus
Mostly present in cytosol (PubMed:7782328).
A minor proportion is mitochondrial (PubMed:7782328).
A very small amount of the protein is associated with nuclei (PubMed:7782328).
Isoform p26
Mitochondrion matrix

PTM

The N-terminus is blocked.

Lau, H. C., Yuan, X., Huang, H., Zhang, M., Hsueh, C. Y., & Gong, H. (2023). Fusobacterium nucleatum facilitates proliferation and autophagy by activating miR-361-3p/NUDT1 axis through oxidative stress in hypopharyngeal squamous cell carcinoma. BMC cancer, 23(1), 990.

Lin, Y., Zhang, F., Jin, Y., Zhong, Q., Tan, W., Liu, J., & Wu, Z. (2022). NUDT1 could be a prognostic biomarker and correlated with immune infiltration in clear cell renal cell carcinoma. Applied bionics and biomechanics, 2022.

Huang, B., Lyu, Z., Qian, Q., Chen, Y., Zhang, J., Li, B., ... & Ma, X. (2022). NUDT1 promotes the accumulation and longevity of CD103+ TRM cells in primary biliary cholangitis. Journal of hepatology, 77(5), 1311-1324.

Shi, J., Xiong, Z., Wang, K., Yuan, C., Huang, Y., Xiao, W., ... & Zhang, X. (2021). HIF2α promotes tumour growth in clear cell renal cell carcinoma by increasing the expression of NUDT1 to reduce oxidative stress. Clinical and translational medicine, 11(11), e592.

Bialkowski, K., & Szpila, A. (2021). Specific 8-oxo-dGTPase activity of MTH1 (NUDT1) protein as a quantitative marker and prognostic factor in human colorectal cancer. Free Radical Biology and Medicine, 176, 257-264.

Vitry, G., Paulin, R., Grobs, Y., Lampron, M. C., Shimauchi, T., Lemay, S. E., ... & Bonnet, S. (2021). Oxidized DNA precursors cleanup by NUDT1 contributes to vascular remodeling in pulmonary arterial hypertension. American Journal of Respiratory and Critical Care Medicine, 203(5), 614-627.

Agarwal, S., & de Jesus Perez, V. A. (2021). In defense of the Nucleus: NUDT1 and oxidative DNA damage in pulmonary arterial Hypertension. American Journal of Respiratory and Critical Care Medicine, 203(5), 541-542.

Shen, Y., Zhang, L., Piao, S., Li, L., Li, J., Xia, Y., ... & Saiyin, W. (2020). NUDT1: A potential independent predictor for the prognosis of patients with oral squamous cell carcinoma. Journal of Oral Pathology & Medicine, 49(3), 210-218.

Bialkowski, K., & Kasprzak, K. S. (2020). A profile of 8-oxo-dGTPase activities in the NCI-60 human cancer panel: meta-analytic insight into the regulation and role of MTH1 (NUDT1) gene expression in carcinogenesis. Free Radical Biology and Medicine, 148, 1-21.

Tahara, Y. K., Kietrys, A. M., Hebenbrock, M., Lee, Y., Wilson, D. L., & Kool, E. T. (2019). Dual inhibitors of 8-oxoguanine surveillance by OGG1 and NUDT1. ACS chemical biology, 14(12), 2606-2615.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

NUDT1 Matched Antibody Pair (824) (CAT#: APMAB-824LY)

Antibody Pairs

NUDT1 Matched Antibody Pair (824) (CAT#: APMAB-824LY)

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Mouse Anti-NUDT1 Recombinant Antibody (5F11) (CAT#: CBMAB-N3863-WJ)

NUDT1 Matched Antibody Pair (824) (CAT#: APMAB-824LY)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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