Human PAPPA2 ELISA Kit (V2LY-0626-LY4998)

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Tested Data
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.215 ng/mL
Detection Range
0.5-100 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
198.5 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Pappalysin 2
Function
Metalloproteinase which specifically cleaves insulin-like growth factor binding protein (IGFBP)-5 at the '163-Ser-|-Lys-164' bond. Shows limited proteolysis toward IGFBP-3.
Biological Process
Bone morphogenesisIEA:Ensembl
Cellular protein metabolic processTAS:Reactome
ProteolysisManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of cell growth1 PublicationNAS:UniProtKB
Response to salt stressIEA:Ensembl
Cellular Location
Secreted
Involvement in disease
Short stature, Dauber-Argente type (SSDA):
An autosomal recessive disorder characterized by progressive postnatal growth failure, moderate microcephaly, thin long bones, mildly decreased bone density, and elevated serum levels of total IGF1, IGFBP3 and IGFBP5. Levels of circulating free IGF1 are reduced.

Xie, Q., Zhang, Z., Chen, Z., Sun, J., Li, M., Wang, Q., & Pan, Y. (2023). Integration of selection signatures and protein interactions reveals NR6A1, PAPPA2, and PIK3C2B as the promising candidate genes underlying the characteristics of licha black pig. Biology, 12(4), 500.

Upners, E. N., Ljubicic, M. L., Busch, A. S., Fischer, M. B., Almstrup, K., Petersen, J. H., ... & Juul, A. (2022). Dynamic changes in serum IGF-I and growth during infancy: associations to body fat, target height, and PAPPA2 genotype. The Journal of Clinical Endocrinology & Metabolism, 107(1), 219-229.

Dong, Y., Zhao, L., Duan, J., Bai, H., Chen, D., Li, S., ... & Wang, Z. (2022). PAPPA2 mutation as a novel indicator stratifying beneficiaries of immune checkpoint inhibitors in skin cutaneous melanoma and non‐small cell lung cancer. Cell Proliferation, 55(9), e13283.

Qin, M., Liang, Z., Qin, H., Huo, Y., Wu, Q., Yang, H., & Tang, G. (2021). Novel prognostic biomarkers in gastric cancer: CGB5, MKNK2, and PAPPA2. Frontiers in oncology, 11, 683582.

Rubio, L., Vargas, A., Rivera, P., López-Gambero, A. J., Tovar, R., Christians, J. K., ... & Suárez, J. (2021). Recombinant IGF-1 induces sex-specific changes in bone composition and remodeling in adult mice with Pappa2 deficiency. International Journal of Molecular Sciences, 22(8), 4048.

Harsanyi, S., Zamborsky, R., Krajciova, L., Kokavec, M., & Danisovic, L. (2021). Genetic study of IL6, GDF5 and PAPPA2 in association with developmental dysplasia of the hip. Genes, 12(7), 986.

Barrios, V., Chowen, J. A., Martín-Rivada, Á., Guerra-Cantera, S., Pozo, J., Yakar, S., ... & Argente, J. (2021). Pregnancy-associated plasma protein (PAPP)-A2 in physiology and disease. Cells, 10(12), 3576.

Fujimoto, M., Andrew, M., & Dauber, A. (2020). Disorders caused by genetic defects associated with GH-dependent genes: PAPPA2 defects. Molecular and cellular endocrinology, 518, 110967.

Christians, J. K., Amiri, N., Schipilow, J. D., Zhang, S. W., & May-Rashke, K. I. (2019). Pappa2 deletion has sex-and age-specific effects on bone in mice. Growth Hormone & IGF Research, 44, 6-10.

Chen, Y., Li, L., Wang, E., Zhang, L., & Zhao, Q. (2019). Abnormal expression of Pappa2 gene may indirectly affect mouse hip development through the IGF signaling pathway. Endocrine, 65, 440-450.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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