Human Recombinant PAPPA2 protein, His Tag (V2LY-0526-LY5963)

Name: Human Recombinant PAPPA2 protein, His Tag
SKU: V2LY-0526-LY5963
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant PAPPA2 protein, His Tag consist of Amino Acid: 234-1396 and predicts a molecular mass of 131 kDa.

Molecule Mass

131 kDa

Sequence

Amino Acid: 234-1396

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Pappalysin 2

Function

Metalloproteinase which specifically cleaves insulin-like growth factor binding protein (IGFBP)-5 at the '163-Ser-|-Lys-164' bond. Shows limited proteolysis toward IGFBP-3.

Biological Process

Bone morphogenesisIEA:Ensembl
Cellular protein metabolic processTAS:Reactome
ProteolysisManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of cell growth1 PublicationNAS:UniProtKB
Response to salt stressIEA:Ensembl

Cellular Location

Secreted

Involvement in disease

Short stature, Dauber-Argente type (SSDA):
An autosomal recessive disorder characterized by progressive postnatal growth failure, moderate microcephaly, thin long bones, mildly decreased bone density, and elevated serum levels of total IGF1, IGFBP3 and IGFBP5. Levels of circulating free IGF1 are reduced.

Xie, Q., Zhang, Z., Chen, Z., Sun, J., Li, M., Wang, Q., & Pan, Y. (2023). Integration of selection signatures and protein interactions reveals NR6A1, PAPPA2, and PIK3C2B as the promising candidate genes underlying the characteristics of licha black pig. Biology, 12(4), 500.

Upners, E. N., Ljubicic, M. L., Busch, A. S., Fischer, M. B., Almstrup, K., Petersen, J. H., ... & Juul, A. (2022). Dynamic changes in serum IGF-I and growth during infancy: associations to body fat, target height, and PAPPA2 genotype. The Journal of Clinical Endocrinology & Metabolism, 107(1), 219-229.

Dong, Y., Zhao, L., Duan, J., Bai, H., Chen, D., Li, S., ... & Wang, Z. (2022). PAPPA2 mutation as a novel indicator stratifying beneficiaries of immune checkpoint inhibitors in skin cutaneous melanoma and non‐small cell lung cancer. Cell Proliferation, 55(9), e13283.

Qin, M., Liang, Z., Qin, H., Huo, Y., Wu, Q., Yang, H., & Tang, G. (2021). Novel prognostic biomarkers in gastric cancer: CGB5, MKNK2, and PAPPA2. Frontiers in oncology, 11, 683582.

Rubio, L., Vargas, A., Rivera, P., López-Gambero, A. J., Tovar, R., Christians, J. K., ... & Suárez, J. (2021). Recombinant IGF-1 induces sex-specific changes in bone composition and remodeling in adult mice with Pappa2 deficiency. International Journal of Molecular Sciences, 22(8), 4048.

Harsanyi, S., Zamborsky, R., Krajciova, L., Kokavec, M., & Danisovic, L. (2021). Genetic study of IL6, GDF5 and PAPPA2 in association with developmental dysplasia of the hip. Genes, 12(7), 986.

Barrios, V., Chowen, J. A., Martín-Rivada, Á., Guerra-Cantera, S., Pozo, J., Yakar, S., ... & Argente, J. (2021). Pregnancy-associated plasma protein (PAPP)-A2 in physiology and disease. Cells, 10(12), 3576.

Fujimoto, M., Andrew, M., & Dauber, A. (2020). Disorders caused by genetic defects associated with GH-dependent genes: PAPPA2 defects. Molecular and cellular endocrinology, 518, 110967.

Christians, J. K., Amiri, N., Schipilow, J. D., Zhang, S. W., & May-Rashke, K. I. (2019). Pappa2 deletion has sex-and age-specific effects on bone in mice. Growth Hormone & IGF Research, 44, 6-10.

Chen, Y., Li, L., Wang, E., Zhang, L., & Zhao, Q. (2019). Abnormal expression of Pappa2 gene may indirectly affect mouse hip development through the IGF signaling pathway. Endocrine, 65, 440-450.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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