Human Recombinant ASGR1 protein, His Tag (V2LY-0526-LY2183)

Name: Human Recombinant ASGR1 protein, His Tag
SKU: V2LY-0526-LY2183
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant ASGR1 protein, His Tag consist of Amino Acid: 62-291 and predicts a molecular mass of 28.8 kDa.

Molecule Mass

28.8 kDa

Verified

HPLC

Sequence

Amino Acid: 62-291

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC. ≥95% as determined by SEC-MALS(Routinely tested).

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Asialoglycoprotein Receptor 1

Function

Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.

Biological Process

Cellular response to extracellular stimulus Source: Ensembl
Protein N-linked glycosylation via asparagine Source: Reactome
Receptor-mediated endocytosis Source: ProtInc
Viral process Source: UniProtKB-KW

Cellular Location

Isoform H1a: Membrane
Isoform H1b: Secreted

Topology

Cytoplasmic: 1-40 aa
Helical: 41-61 aa
Extracellular: 62-291 aa

PTM

Phosphorylated on a cytoplasmic Ser residue.

Susan-Resiga, D., Girard, E., Essalmani, R., Roubtsova, A., Marcinkiewicz, J., Derbali, M. R., ... & Seidah, N. G. (2021). Asialoglycoprotein receptor 1 is a novel PCSK9-independent ligand of liver LDLRthat is shed by Furin. bioRxiv.

Zhao, X., Tang, B., Shi, Z. H., Deng, Z. W., Deng, Y., Tan, L. M., ... & Qiu, C. F. (2020). Asialoglycoprotein receptor 1 gene expression in peripheral blood monocytes associates with serum total cholesterol and low-density lipoprotein cholesterol levels. Chinese medical journal, 133(12), 1505.

Janiszewski, M., Sohn, W., Su, C., Hsu, Y. H., Finger, E., & Kaufman, A. (2019). A RANDOMIZED, PLACEBO-CONTROLLED, DOUBLE-BLIND, ASCENDING SINGLE-DOSE, PHASE 1 STUDY TO EVALUATE THE SAFETY, TOLERABILITY, PHARMACOKINETICS, AND PHARMACODYNAMICS OF AMG 529, A NOVEL ANTI-ASGR1 MONOCLONAL ANTIBODY, IN HEALTHY SUBJECTS. Journal of the American College of Cardiology, 73(9S1), 1755-1755.

Tanowitz, M., Hettrick, L., Revenko, A., Kinberger, G. A., Prakash, T. P., & Seth, P. P. (2017). Asialoglycoprotein receptor 1 mediates productive uptake of N-acetylgalactosamine-conjugated and unconjugated phosphorothioate antisense oligonucleotides into liver hepatocytes. Nucleic acids research, 45(21), 12388-12400.

Chen, C., Li, K., Jiang, H., Song, F., Gao, H., Pan, X., ... & Li, Z. (2017). Development of T cells carrying two complementary chimeric antigen receptors against glypican-3 and asialoglycoprotein receptor 1 for the treatment of hepatocellular carcinoma. Cancer Immunology, Immunotherapy, 66(4), 475-489.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus Recombinant ASGR1 protein, ECD, His Tag (CAT#: V2LY-0526-LY1378)

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Human Recombinant ASGR1 protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY2182)

Mouse Recombinant ASGR1 protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY7828)

Mouse Recombinant ASGR1 protein, His Tag (CAT#: V2LY-0526-LY7829)

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Human Recombinant ASGR1 protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY2181)

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Mouse Recombinant ASGR1 protein, His Tag (CAT#: V2LY-0526-LY7829)

Human Recombinant ASGR1 protein, Biotin Conjugated, His Tag (CAT#: V2LY-0526-LY2182)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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