Human Recombinant CCDC134 protein, His Tag (V2LY-0526-LY2619)

Name: Human Recombinant CCDC134 protein, His Tag
SKU: V2LY-0526-LY2619
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CCDC134 protein, His Tag consist of Amino Acid: 1-229 and predicts a molecular mass of 25.7 kDa.

Molecule Mass

25.7 kDa

Sequence

Amino Acid: 1-229

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>98% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Coiled-Coil Domain Containing 134

Function

In extracellular secreted form, promotes proliferation and activation of CD8+ T cells, suggesting a cytokine-like function (PubMed:25125657).
Enhances cytotoxic anti-tumor activity of CD8+ T cells (PubMed:25125657).
May inhibit ERK and JNK signaling activity (PubMed:18087676, PubMed:23070808).
May suppress cell migration and invasion activity, via its effects on ERK and JNK signaling (PubMed:23070808).
In the nucleus, enhances stability of the PCAF histone acetyltransferase (HAT) complex member TADA2A and thus promotes PCAF-mediated H3K14 and H4K8 HAT activity. May inhibit TADA2A-mediated TP53/p53 'Lys-321' acetylation, leading to reduced TP53 stability and transcriptional activity. May also promote TADA2A-mediated XRCC6 acetylation thus facilitating cell apoptosis in response to DNA damage.

Biological Process

Angiogenesis Source: Ensembl
Embryonic hemopoiesis Source: Ensembl
Embryonic liver development Source: Ensembl
Placenta development Source: Ensembl
Ventricular system development Source: Ensembl

Cellular Location

Endoplasmic reticulum; Cytoplasm; Secreted; Nucleus. Accumulates in the nucleus in response to UV irradiation (PubMed:22644376).

PTM

O-glycosylated, with additional sialic acid modifications.

Yin, S., Liao, Q., Wang, Y., Shi, Q., Xia, P., Yi, M., & Huang, J. (2021). Ccdc134 deficiency impairs cerebellar development and motor coordination. Genes, Brain and Behavior, 20(7), e12763.

Holick, M. F., Shirvani, A., & Charoenngam, N. (2021). Fetal Fractures in an Infant with Maternal Ehlers-Danlos Syndrome, CCDC134 Pathogenic Mutation and a Negative Genetic Test for Osteogenesis Imperfecta. Children, 8(6), 512.

Dubail, J., Brunelle, P., Baujat, G., Huber, C., Doyard, M., Michot, C., ... & Cormier‐Daire, V. (2020). Homozygous loss‐of‐function mutations in CCDC134 are responsible for a severe form of osteogenesis imperfecta. Journal of Bone and Mineral Research, 35(8), 1470-1480.

Xia, P., Gong, X., Xiao, L., Wang, Y., Zhang, T., Liao, Q., ... & Huang, J. (2018). CCDC134 ameliorated experimental autoimmune encephalomyelitis by suppressing Th1 and Th17 cells. Brain, behavior, and immunity, 71, 158-168.

Yu, B., Zhang, T., Xia, P., Gong, X., Qiu, X., & Huang, J. (2018). CCDC134 serves a crucial role in embryonic development. International journal of molecular medicine, 41(1), 381-390.

Xia, P., Zhang, T., Gong, X., Xiao, L., Liao, Q., Qiu, X., & Huang, J. (2017). Amelioration of adjuvant-induced arthritis in CCDC134-overexpressing transgenic mice. Biochemical and biophysical research communications, 490(2), 111-116.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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