Human Recombinant CD52 protein, Biotin Conjugated, hFc & AVI Tag (V2LY-0526-LY2899)

Name: Human Recombinant CD52 protein, Biotin Conjugated, hFc & AVI Tag
SKU: V2LY-0526-LY2899
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

hFc & AVI Tag

Protein Construction

This product is Human Recombinant CD52 protein, Biotin Conjugated, hFc & AVI Tag consist of Amino Acid: 1-36 and predicts a molecular mass of 29.7 kDa.

Molecule Mass

29.7 kDa

Conjugates

Biotin

Sequence

Amino Acid: 1-36

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD52 Molecule

Function

May play a role in carrying and orienting carbohydrate, as well as having a more specific role.

Biological Process

Positive regulation of cytosolic calcium ion concentration Source: UniProtKB
Respiratory burst Source: UniProtKB

Cellular Location

Cell membrane

Frota, N. F., de Sousa Rebouças, A., Fuzo, C. A., & Lourenzoni, M. R. (2021). Alemtuzumab scFv fragments and CD52 interaction study through molecular dynamics simulation and binding free energy. Journal of Molecular Graphics and Modelling, 107949.

Mao, R., Yang, F., Zhang, Y., Liu, H., Guo, P., Liu, Y., & Zhang, T. (2021). High expression of CD52 in adipocytes: a potential therapeutic target for obesity with type 2 diabetes. Aging (Albany NY), 13(8), 11043.

Wang, J., Zhang, G., Sui, Y., Yang, Z., Chu, Y., Tang, H., ... & Wu, C. (2020). CD52 Is a Prognostic Biomarker and Associated With Tumor Microenvironment in Breast Cancer. Frontiers in Genetics, 11, 1350.

Bhamidipati, K., Silberstein, J. L., Chaichian, Y., Baker, M. C., Lanz, T. V., Zia, A., ... & Robinson, W. H. (2020). CD52 is Elevated on B cells of SLE Patients and Regulates B Cell Function. Frontiers in Immunology, 11, 3736.

Shathili, A. M., Bandala-Sanchez, E., John, A., Goddard-Borger, E. D., Thaysen-Andersen, M., Everest-Dass, A. V., ... & Packer, N. H. (2019). Specific sialoforms required for the immune suppressive activity of human soluble CD52. Frontiers in immunology, 10, 1967.

Zheng, C., Zhuang, C., Chen, Y., Fu, Q., Qian, H., Wang, Y., ... & Qi, N. (2018). Improved process robustness, product quality and biological efficacy of an anti-CD52 monoclonal antibody upon pH shift in Chinese hamster ovary cell perfusion culture. Process Biochemistry, 65, 123-129.

Simon, M., Ipek, R., Homola, G. A., Rovituso, D. M., Schampel, A., Kleinschnitz, C., & Kuerten, S. (2018). Anti-CD52 antibody treatment depletes B cell aggregates in the central nervous system in a mouse model of multiple sclerosis. Journal of neuroinflammation, 15(1), 1-15.

Rashidi, M., Bandala-Sanchez, E., Lawlor, K. E., Zhang, Y., Neale, A. M., Vijayaraj, S. L., ... & Harrison, L. C. (2018). CD52 inhibits Toll-like receptor activation of NF-κ B and triggers apoptosis to suppress inflammation. Cell Death & Differentiation, 25(2), 392-405.

Bandala-Sanchez, E., Bediaga, N. G., Goddard-Borger, E. D., Ngui, K., Naselli, G., Stone, N. L., ... & Harrison, L. C. (2018). CD52 glycan binds the proinflammatory B box of HMGB1 to engage the Siglec-10 receptor and suppress human T cell function. Proceedings of the National Academy of Sciences, 115(30), 7783-7788.

Zhao, Y., Su, H., Shen, X., Du, J., Zhang, X., & Zhao, Y. (2017). The immunological function of CD52 and its targeting in organ transplantation. Inflammation Research, 66(7), 571-578.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus Recombinant CD52 protein, hFc Tag (CAT#: V2LY-0526-LY1442)

Human Recombinant CD52 protein, hFc Tag (CAT#: V2LY-0526-LY2900)

Mouse Recombinant CD52 protein, hFc Tag (CAT#: V2LY-0526-LY8041)

Rat Recombinant CD52 protein, hFc Tag (CAT#: V2LY-0526-LY9253)

Mouse Cd52 ELISA Kit (CAT#: V2LY-0626-LY2110)

Human CD52 ELISA Kit (CAT#: V2LY-0626-LY5546)

Related Products

Rat Recombinant CD52 protein, hFc Tag (CAT#: V2LY-0526-LY9253)

Human Recombinant CD52 protein, hFc Tag (CAT#: V2LY-0526-LY2900)

Human CD52 ELISA Kit (CAT#: V2LY-0626-LY5546)

Mouse Anti-CD52 Recombinant Antibody (HI186) (CAT#: CBMAB-C0703-CQ)

Rat Anti-CD52 Recombinant Antibody (YTH 34.5-G2b) (CAT#: CBMAB-C10474-LY)

Human Anti-CD52 Recombinant Antibody (CBXC-0724) (CAT#: CBMAB-C1838-CQ)

Mouse Recombinant CD52 protein, hFc Tag (CAT#: V2LY-0526-LY8041)

Mouse Cd52 ELISA Kit (CAT#: V2LY-0626-LY2110)

Rabbit Anti-CD52 Recombinant Antibody (C0048) (CAT#: CBMAB-C0048-CN)

Cynomolgus Recombinant CD52 protein, hFc Tag (CAT#: V2LY-0526-LY1442)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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