Mouse Cd52 ELISA Kit (V2LY-0626-LY2110)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.0073 ng/mL
Detection Range
0.015-3 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
7.8 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
CD52 Molecule
Function
May play a role in carrying and orienting carbohydrate, as well as having a more specific role.
Biological Process
Positive regulation of cytosolic calcium ion concentration Source: UniProtKB
Respiratory burst Source: UniProtKB
Cellular Location
Cell membrane

Frota, N. F., de Sousa Rebouças, A., Fuzo, C. A., & Lourenzoni, M. R. (2021). Alemtuzumab scFv fragments and CD52 interaction study through molecular dynamics simulation and binding free energy. Journal of Molecular Graphics and Modelling, 107949.

Mao, R., Yang, F., Zhang, Y., Liu, H., Guo, P., Liu, Y., & Zhang, T. (2021). High expression of CD52 in adipocytes: a potential therapeutic target for obesity with type 2 diabetes. Aging (Albany NY), 13(8), 11043.

Wang, J., Zhang, G., Sui, Y., Yang, Z., Chu, Y., Tang, H., ... & Wu, C. (2020). CD52 Is a Prognostic Biomarker and Associated With Tumor Microenvironment in Breast Cancer. Frontiers in Genetics, 11, 1350.

Bhamidipati, K., Silberstein, J. L., Chaichian, Y., Baker, M. C., Lanz, T. V., Zia, A., ... & Robinson, W. H. (2020). CD52 is Elevated on B cells of SLE Patients and Regulates B Cell Function. Frontiers in Immunology, 11, 3736.

Shathili, A. M., Bandala-Sanchez, E., John, A., Goddard-Borger, E. D., Thaysen-Andersen, M., Everest-Dass, A. V., ... & Packer, N. H. (2019). Specific sialoforms required for the immune suppressive activity of human soluble CD52. Frontiers in immunology, 10, 1967.

Zheng, C., Zhuang, C., Chen, Y., Fu, Q., Qian, H., Wang, Y., ... & Qi, N. (2018). Improved process robustness, product quality and biological efficacy of an anti-CD52 monoclonal antibody upon pH shift in Chinese hamster ovary cell perfusion culture. Process Biochemistry, 65, 123-129.

Simon, M., Ipek, R., Homola, G. A., Rovituso, D. M., Schampel, A., Kleinschnitz, C., & Kuerten, S. (2018). Anti-CD52 antibody treatment depletes B cell aggregates in the central nervous system in a mouse model of multiple sclerosis. Journal of neuroinflammation, 15(1), 1-15.

Rashidi, M., Bandala-Sanchez, E., Lawlor, K. E., Zhang, Y., Neale, A. M., Vijayaraj, S. L., ... & Harrison, L. C. (2018). CD52 inhibits Toll-like receptor activation of NF-κ B and triggers apoptosis to suppress inflammation. Cell Death & Differentiation, 25(2), 392-405.

Bandala-Sanchez, E., Bediaga, N. G., Goddard-Borger, E. D., Ngui, K., Naselli, G., Stone, N. L., ... & Harrison, L. C. (2018). CD52 glycan binds the proinflammatory B box of HMGB1 to engage the Siglec-10 receptor and suppress human T cell function. Proceedings of the National Academy of Sciences, 115(30), 7783-7788.

Zhao, Y., Su, H., Shen, X., Du, J., Zhang, X., & Zhao, Y. (2017). The immunological function of CD52 and its targeting in organ transplantation. Inflammation Research, 66(7), 571-578.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus Recombinant CD52 protein, hFc Tag (CAT#: V2LY-0526-LY1442)

Human Recombinant CD52 protein, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY2899)

Human Recombinant CD52 protein, hFc Tag (CAT#: V2LY-0526-LY2900)

Mouse Recombinant CD52 protein, hFc Tag (CAT#: V2LY-0526-LY8041)

Rat Recombinant CD52 protein, hFc Tag (CAT#: V2LY-0526-LY9253)

Human CD52 ELISA Kit (CAT#: V2LY-0626-LY5546)

Related Products

Human CD52 ELISA Kit (CAT#: V2LY-0626-LY5546)

Mouse Anti-CD52 Recombinant Antibody (HI186) (CAT#: CBMAB-C0703-CQ)

Cynomolgus Recombinant CD52 protein, hFc Tag (CAT#: V2LY-0526-LY1442)

Rabbit Anti-CD52 Recombinant Antibody (C0048) (CAT#: CBMAB-C0048-CN)

Mouse Recombinant CD52 protein, hFc Tag (CAT#: V2LY-0526-LY8041)

Rabbit Anti-CD52 Recombinant Antibody (CBXS-2338) (CAT#: CBMAB-S5097-CQ)

Human Recombinant CD52 protein, hFc Tag (CAT#: V2LY-0526-LY2900)

Human Anti-CD52 Recombinant Antibody (CBXC-0724) (CAT#: CBMAB-C1838-CQ)

Mouse Anti-CD52 Recombinant Antibody (CBFYC-1354) (CAT#: CBMAB-C1412-FY)

Rat Recombinant CD52 protein, hFc Tag (CAT#: V2LY-0526-LY9253)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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