Human Recombinant CES2 protein, His Tag (V2LY-0526-LY2571)

Name: Human Recombinant CES2 protein, His Tag
SKU: V2LY-0526-LY2571
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CES2 protein, His Tag consist of Amino Acid: 1-559 and predicts a molecular mass of 60.4 kDa.

Molecule Mass

60.4 kDa

Verified

HPLC

Sequence

Amino Acid: 1-559

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Ceramide Synthase 2

Function

Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs (PubMed:9169443).
Shows high catalytic efficiency for hydrolysis of cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine (PubMed:9169443).
Hydrolyzes aspirin, substrates with large alcohol group and small acyl group and endogenous lipids such as triacylglycerol (PubMed:28677105).
Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes of 2-arachidonoylglycerol and prostaglandins (PubMed:21049984).

Biological Process

Catabolic process Source: ProtInc
Lipid catabolic process Source: GO_Central
Prostaglandin metabolic process Source: BHF-UCL
Xenobiotic metabolic process Source: Reactome

Cellular Location

Endoplasmic reticulum lumen

PTM

Glycosylated.

Xu, Y., Pan, X., Hu, S., Zhu, Y., Cassim Bawa, F., Li, Y., ... & Zhang, Y. (2021). Hepatocyte-specific expression of human carboxylesterase 2 attenuates nonalcoholic steatohepatitis in mice. American Journal of Physiology-Gastrointestinal and Liver Physiology, 320(2), G166-G174.

Shen, Y., Eades, W., & Yan, B. (2021). Remdesivir potently inhibits carboxylesterase‐2 through covalent modifications: signifying strong drug‐drug interactions. Fundamental & Clinical Pharmacology, 35(2), 432-434.

Chalhoub, G., Kolleritsch, S., Maresch, L. K., Taschler, U., Pajed, L., Tilp, A., ... & Haemmerle, G. (2021). Carboxylesterase 2 proteins are efficient diglyceride and monoglyceride lipases possibly implicated in metabolic disease. Journal of lipid research, 62.

Song, Y. Q., He, R. J., Pu, D., Guan, X. Q., Shi, J. H., Li, Y. G., ... & Ge, G. B. (2021). Discovery and Characterization of the Biflavones From Ginkgo biloba as Highly Specific and Potent Inhibitors Against Human Carboxylesterase 2. Frontiers in pharmacology, 12.

Qian, X. K., Zhang, J., Song, P. F., Zhao, Y. S., Ma, H. Y., Jin, Q., ... & Zou, L. W. (2021). Discovery of pyrazolones as novel carboxylesterase 2 inhibitors that potently inhibit the adipogenesis in cells. Bioorganic & Medicinal Chemistry, 40, 116187.

Tian, X., Yan, F., Zheng, J., Cui, X., Feng, L., Li, S., ... & Ma, X. (2019). Endoplasmic reticulum targeting ratiometric fluorescent probe for carboxylesterase 2 detection in drug-induced acute liver injury. Analytical chemistry, 91(24), 15840-15845.

Yi, J., Bai, R., An, Y., Liu, T. T., Liang, J. H., Tian, X. G., ... & Zhang, H. L. (2019). A natural inhibitor from Alisma orientale against human carboxylesterase 2: kinetics, circular dichroism spectroscopic analysis, and docking simulation. International journal of biological macromolecules, 133, 184-189.

Zhao, Y. S., Ruan, H. L., Wang, X. Y., Chen, C., Song, P. F., Lü, C. W., & Zou, L. W. (2019). Catalyst-free visible-light-induced condensation to synthesize bis (indolyl) methanes and biological activity evaluation of them as potent human carboxylesterase 2 inhibitors. RSC Advances, 9(68), 40168-40175.

Ruby, M. A., Massart, J., Hunerdosse, D. M., Schönke, M., Correia, J. C., Louie, S. M., ... & Zierath, J. R. (2017). Human carboxylesterase 2 reverses obesity-induced diacylglycerol accumulation and glucose intolerance. Cell reports, 18(3), 636-646.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Recombinant CES2 protein, His Tag (CAT#: V2LY-0526-LY7913)

Human Recombinant CES2, Active protein, GST Tag (CAT#: V2LY-0526-LY2570)

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Isotype control

For research use only. Not intended for any clinical use.

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