Human Recombinant JTB protein, Fc Tag (V2LY-0526-LY5115)

Name: Human Recombinant JTB protein, Fc Tag
SKU: V2LY-0526-LY5115
Rating: 5 (1 reviews)

See all JTB antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

Fc Tag

Protein Construction

This product is Human Recombinant JTB protein, Fc Tag consist of Amino Acid: 1-105 and predicts a molecular mass of 34.7 kDa.

Molecule Mass

34.7 kDa

Sequence

Amino Acid: 1-105

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

jumping translocation breakpoint

Function

Required for normal cytokinesis during mitosis. Plays a role in the regulation of cell proliferation. May be a component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Increases AURKB activity. Inhibits apoptosis induced by TGFB1 (By similarity).
Overexpression induces swelling of mitochondria and reduces mitochondrial membrane potential (By similarity).

Biological Process

Apoptotic processIEA:UniProtKB-KW
Mitotic cell cycleManual Assertion Based On ExperimentIMP:UniProtKB
Mitotic cytokinesisManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of protein kinase activityManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Membrane; Mitochondrion; Cytoplasm; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cytoskeleton, spindle. Detected at the centrosome and along spindle fibers during prophase and metaphase. Detected at the midbody during telophase.

Topology

Extracellular: 31-105
Helical: 106-126
Cytoplasmic: 127-146

Jayathirtha, M., Jayaweera, T., Whitham, D., Petre, B. A., Neagu, A. N., & Darie, C. C. (2023). Two-Dimensional Polyacrylamide Gel Electrophoresis Coupled with Nanoliquid Chromatography–Tandem Mass Spectrometry-Based Identification of Differentially Expressed Proteins and Tumorigenic Pathways in the MCF7 Breast Cancer Cell Line Transfected for Jumping Translocation Breakpoint Protein Overexpression. International Journal of Molecular Sciences, 24(19), 14714.

Jayathirtha, M., Neagu, A. N., Whitham, D., Alwine, S., & Darie, C. C. (2022). Investigation of the effects of overexpression of jumping translocation breakpoint (JTB) protein in MCF7 cells for potential use as a biomarker in breast cancer. American Journal of Cancer Research, 12(4), 1784.

Jayathirtha, M., Neagu, A. N., Whitham, D., Alwine, S., & Darie, C. C. (2022). Investigation of the effects of downregulation of jumping translocation breakpoint (JTB) protein expression in MCF7 cells for potential use as a biomarker in breast cancer. American Journal of Cancer Research, 12(9), 4373.

Jayathirtha, M., Whitham, D., Alwine, S., Donnelly, M., Neagu, A. N., & Darie, C. C. (2022). Investigating the Function of Human Jumping Translocation Breakpoint Protein (hJTB) and Its Interacting Partners through In-Solution Proteomics of MCF7 Cells. Molecules, 27(23), 8301.

Jayathirtha, M., Whitham, D., Channaveerappa, D., & Darie, C. (2022). Mass Spectrometry based Proteomics to Investigate and Characterize the Human Jumping Translocation Breakpoint (hJTB) Protein using Cancer Cell Lines. The FASEB Journal, 36.

Jayathirtha, M., Channaveerappa, D., & Darie, C. (2021). Investigation and Characterization of the Jumping Translocation Breakpoint (JTB) Protein using Mass Spectrometry based Proteomics. The FASEB Journal, 35.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human Recombinant JTB protein, Fc Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human JTB ELISA Kit (CAT#: V2LY-0626-LY5102)

Related Products

Mouse Anti-JTB Recombinant Antibody (CBFYJ-011) (CAT#: CBMAB-0023-FY)

Rabbit Anti-JTB Recombinant Antibody (EG1723) (CAT#: CBMAB-EN2061-LY)

Mouse Anti-JTB Recombinant Antibody (CBFYJ-014) (CAT#: CBMAB-0026-FY)

Mouse Anti-JTB Recombinant Antibody (4E7) (CAT#: CBMAB-0215-WJ)

Human JTB ELISA Kit (CAT#: V2LY-0626-LY5102)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry