Human Recombinant PRDX5 protein, His Tag (V2LY-0526-LY6114)

Name: Human Recombinant PRDX5 protein, His Tag
SKU: V2LY-0526-LY6114
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant PRDX5 protein, His Tag consist of Amino Acid: 53-214 and predicts a molecular mass of 18.5 kDa.

Molecule Mass

18.5 kDa

Sequence

Amino Acid: 53-214

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile HEPES, NaCl

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

PRDX5

Function

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events.

Biological Process

Cell redox homeostasisManual Assertion Based On ExperimentIBA:GO_Central
Cellular response to oxidative stressManual Assertion Based On ExperimentIBA:GO_Central
Cellular response to reactive oxygen speciesManual Assertion Based On ExperimentIMP:UniProtKB
Hydrogen peroxide catabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Inflammatory responseManual Assertion Based On ExperimentTAS:UniProtKB
NADPH oxidationIDA:UniProtKB
Negative regulation of apoptotic processManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of oxidoreductase activityIDA:UniProtKB
Negative regulation of transcription by RNA polymerase IIIManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of collagen biosynthetic processIDA:UniProtKB
Reactive nitrogen species metabolic processIDA:UniProtKB
Regulation of apoptosis involved in tissue homeostasisIDA:UniProtKB
Response to oxidative stressManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Isoform Mitochondrial
Mitochondrion
Isoform Cytoplasmic+peroxisomal
Cytoplasm
Peroxisome matrix
Imported into peroxisomes via peroxisomal targeting signal 1 receptor PEX5.

PTM

S-palmitoylated (PubMed:31740833).
Palmitoylation occurs on the active site, inhibiting its reactivity; therefore PRDX5 palmitoylation status determines its antioxidant capacity (PubMed:31740833).
Isoform Mitochondrial
S-palmitoylated (PubMed:31740833).
Depalmitoylated by ABHD10 (PubMed:31740833).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rat Prdx5 ELISA Kit (CAT#: V2LY-0626-LY1873)

PRDX5 Matched Antibody Pair (926) (CAT#: APMAB-926LY)

Mouse Recombinant PRDX5 protein, His Tag (CAT#: V2LY-0526-LY8770)

Antibody Pairs

PRDX5 Matched Antibody Pair (926) (CAT#: APMAB-926LY)

Related Products

Mouse Anti-PRDX5 Recombinant Antibody (12A) (CAT#: CBMAB-P2746-YC)

Mouse Anti-PRDX5 Recombinant Antibody (6A10) (CAT#: CBMAB-P2748-YC)

Mouse Anti-PRDX5 Recombinant Antibody (3F11) (CAT#: CBMAB-P2747-YC)

Rat Prdx5 ELISA Kit (CAT#: V2LY-0626-LY1873)

Mouse Recombinant PRDX5 protein, His Tag (CAT#: V2LY-0526-LY8770)

PRDX5 Matched Antibody Pair (926) (CAT#: APMAB-926LY)

Mouse Anti-PRDX5 Recombinant Antibody (3E6) (CAT#: CBMAB-A7014-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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