Rat Prdx5 ELISA Kit (V2LY-0626-LY1873)

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Tested Data
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Basic Information

Sensitivity
0.022 ng/mL
Detection Range
0.05-20 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Rat
Assay Type
Sandwich
Reactivity
Rat
Assay Time
1.5 h
Molecule Mass
22.2 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
PRDX5
Function
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events.
Biological Process
Cell redox homeostasisManual Assertion Based On ExperimentIBA:GO_Central
Cellular response to oxidative stressManual Assertion Based On ExperimentIBA:GO_Central
Cellular response to reactive oxygen speciesManual Assertion Based On ExperimentIMP:UniProtKB
Hydrogen peroxide catabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Inflammatory responseManual Assertion Based On ExperimentTAS:UniProtKB
NADPH oxidationIDA:UniProtKB
Negative regulation of apoptotic processManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of oxidoreductase activityIDA:UniProtKB
Negative regulation of transcription by RNA polymerase IIIManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of collagen biosynthetic processIDA:UniProtKB
Reactive nitrogen species metabolic processIDA:UniProtKB
Regulation of apoptosis involved in tissue homeostasisIDA:UniProtKB
Response to oxidative stressManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Isoform Mitochondrial
Mitochondrion
Isoform Cytoplasmic+peroxisomal
Cytoplasm
Peroxisome matrix
Imported into peroxisomes via peroxisomal targeting signal 1 receptor PEX5.
PTM
S-palmitoylated (PubMed:31740833).
Palmitoylation occurs on the active site, inhibiting its reactivity; therefore PRDX5 palmitoylation status determines its antioxidant capacity (PubMed:31740833).
Isoform Mitochondrial
S-palmitoylated (PubMed:31740833).
Depalmitoylated by ABHD10 (PubMed:31740833).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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