Human Recombinant PTPN12 protein, GST Tag (V2LY-0526-LY6410)

Name: Human Recombinant PTPN12 protein, GST Tag
SKU: V2LY-0526-LY6410
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant PTPN12 protein, GST Tag consist of Amino Acid: 1-355 and predicts a molecular mass of 69.4 kDa.

Molecule Mass

69.4 kDa

Sequence

Amino Acid: 1-355

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tirs, NaCl, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

protein tyrosine phosphatase, non-receptor type 12

Function

Dephosphorylates a range of proteins, and thereby regulates cellular signaling cascades (PubMed:18559503).
Dephosphorylates cellular tyrosine kinases, such as ERBB2 and PTK2B/PYK2, and thereby regulates signaling via ERBB2 and PTK2B/PYK2 (PubMed:17329398, PubMed:27134172).
Selectively dephosphorylates ERBB2 phosphorylated at 'Tyr-1112', 'Tyr-1196', and/or 'Tyr-1248' (PubMed:27134172).

Biological Process

Cellular response to epidermal growth factor stimulusManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of ERBB signaling pathwayTAS:Reactome
Negative regulation of platelet-derived growth factor receptor-beta signaling pathwayTAS:Reactome
Peptidyl-tyrosine dephosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Protein dephosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of epidermal growth factor receptor signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Tissue regenerationIEA:Ensembl

Cellular Location

Cytoplasm
Cell junction, focal adhesion
Cell projection, podosome
Partial translocation to focal adhesion sites may be mediated by interaction with SORBS2.

PTM

Phosphorylated by STK24/MST3 and this results in inhibition of its activity.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant PTPN12 protein (CAT#: V2LY-0526-LY6409)

Human Recombinant PTPN12, Active protein, GST Tag (CAT#: V2LY-0526-LY6411)

Related Products

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Mouse Anti-PTPN12 Recombinant Antibody (4G6) (CAT#: CBMAB-P3423-YC)

Mouse Anti-PTPN12 Recombinant Antibody (AG25) (CAT#: CBMAB-P3424-YC)

Rabbit Anti-PTPN12 Recombinant Antibody (CB-5-2F8) (CAT#: CBMAB-BT072LY)

Human Recombinant PTPN12 protein (CAT#: V2LY-0526-LY6409)

Mouse Anti-PTPN12 Recombinant Antibody (F-12) (CAT#: CBMAB-P3425-YC)

Human Recombinant PTPN12, Active protein, GST Tag (CAT#: V2LY-0526-LY6411)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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