Human Recombinant SERPINA12 protein, His Tag (V2LY-0526-LY6717)

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Datasheet
SDS
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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host
HEK293 Cells
Protein Species
Human
Tag
His Tag
Protein Construction
This product is Human Recombinant SERPINA12 protein, His Tag consist of Amino Acid: 1-414 and predicts a molecular mass of 46.5 kDa.
Molecule Mass
46.5 kDa
Verified
HPLC
Sequence
Amino Acid: 1-414
Species
Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
≥97% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.
Endotoxin
Please contact us for more information.
Format
Lyophilized
Reconstitution
Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.
Buffer
Lyophilized from sterile PBS
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
More Infomation

Target

Full Name
Serpin Family A Member 12
Function
Adipokine that modulates insulin action by specifically inhibiting its target protease KLK7 in white adipose tissues.
Biological Process
Biological Process gluconeogenesisIEA:Ensembl
Biological Process lipid biosynthetic processIEA:Ensembl
Biological Process negative regulation of endopeptidase activityManual Assertion Based On ExperimentIBA:GO_Central
Biological Process negative regulation of gluconeogenesisIEA:Ensembl
Biological Process negative regulation of lipid biosynthetic processIEA:Ensembl
Biological Process phosphatidylinositol 3-kinase signalingIEA:Ensembl
Biological Process positive regulation of insulin receptor signaling pathwayIEA:Ensembl
Biological Process positive regulation of phosphatidylinositol 3-kinase signalingIEA:Ensembl
Biological Process regulation of cholesterol metabolic processIEA:Ensembl
Biological Process regulation of triglyceride metabolic processIEA:Ensembl
Cellular Location
Secreted
PTM
Glycosylation slightly decreases affinity for heparin, but otherwise has no significant effect on KLK7 inhibitory activity or thermal stability of the protein.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

SERPINA12 Matched Antibody Pair (1056) (CAT#: APMAB-1056LY)

Antibody Pairs

SERPINA12 Matched Antibody Pair (1056) (CAT#: APMAB-1056LY)

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Mouse Anti-SERPINA12 Recombinant Antibody (CBXS-0226) (CAT#: CBMAB-S3207-CQ)

Mouse Anti-SERPINA12 Recombinant Antibody (VP63) (CAT#: CBMAB-S6673-CQ)

Mouse Anti-SERPINA12 (AA 21-414) Recombinant Antibody (CBXS-0224) (CAT#: CBMAB-S3205-CQ)

Mouse Anti-SERPINA12 Recombinant Antibody (CBXS-0225) (CAT#: CBMAB-S3206-CQ)

Mouse Anti-SERPINA12 Recombinant Antibody (CBXS-3021) (CAT#: CBMAB-S5767-CQ)

SERPINA12 Matched Antibody Pair (1056) (CAT#: APMAB-1056LY)

Mouse Anti-SERPINA12 Recombinant Antibody (CBXS-1836) (CAT#: CBMAB-S4641-CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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