Human Recombinant SSB protein, His Tag-1 (V2LY-0526-LY6950)

Name: Human Recombinant SSB protein, His Tag-1
SKU: V2LY-0526-LY6950
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant SSB protein, His Tag consist of Amino Acid: 1-408 and predicts a molecular mass of 49.2 kDa.

Molecule Mass

49.2 kDa

Sequence

Amino Acid: 1-408

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Sjogren syndrome antigen B (autoantigen La)

Function

Binds to the 3' poly(U) terminus of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation (PubMed:3192525, PubMed:2470590).
In case of Coxsackievirus B3 infection, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed:12384597).

Biological Process

Biological Process histone mRNA metabolic processManual Assertion Based On ExperimentTAS:ProtInc
Biological Process IRES-dependent viral translational initiationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process nuclear histone mRNA catabolic processManual Assertion Based On ExperimentIMP:CAFA
Biological Process protein localization to cytoplasmic stress granuleManual Assertion Based On ExperimentIDA:AgBase
Biological Process tRNA 3'-end processingManual Assertion Based On ExperimentIGI:CAFA
Biological Process tRNA 5'-leader removalManual Assertion Based On ExperimentIGI:CAFA
Biological Process tRNA export from nucleusManual Assertion Based On ExperimentIMP:CAFA
Biological Process tRNA modificationManual Assertion Based On ExperimentTAS:ProtInc
Biological Process tRNA processingManual Assertion Based On ExperimentIMP:CAFA

Cellular Location

Nucleus

PTM

Phosphorylated. The phosphorylation sites are at the C-terminal part of the protein.
The N-terminus is blocked.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rabbit Anti-SSB (Phosphorylated S366) Polyclonal Antibody (PTM-CBPAB-0996YC) (CAT#: PTM-CBPAB-0996YC)

Human Recombinant SSB protein, His Tag (CAT#: V2LY-0526-LY6949)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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