Mouse Esam ELISA Kit (V2LY-0626-LY2334)

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Tested Data
Datasheet
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Basic Information

Sensitivity
0.153 ng/mL
Detection Range
0.3-90 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
41.8 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
endothelial cell adhesion molecule
Research Area
Can mediate aggregation most likely through a homophilic molecular interaction.
Biological Process
Bicellular tight junction assembly Source: ARUK-UCL
Cell-cell adhesion Source: ARUK-UCL
Cellular protein localization Source: ARUK-UCL
Homophilic cell adhesion via plasma membrane adhesion molecules Source: Ensembl
Maintenance of blood-brain barrier Source: ARUK-UCL
Regulation of actin cytoskeleton reorganization Source: ARUK-UCL
Regulation of actin filament polymerization Source: ARUK-UCL
Cellular Location
Cell membrane; Adherens junction; Tight junction
Topology
Extracellular: 30-248
Helical: 249-269
Cytoplasmic: 270-390

Hansmeier, N. R., Büschlen, I. S., Behncke, R. Y., Ulferts, S., Bisoendial, R., & Hägerling, R. (2022). 3D Visualization of Human Blood Vascular Networks Using Single-Domain Antibodies Directed against Endothelial Cell-Selective Adhesion Molecule (ESAM). International journal of molecular sciences, 23(8), 4369.

Buncha, V., Fopiano, K. A., Tian, Y., & Bagi, Z. (2021). Role of Endothelial Cell Specific Adhesion Molecule in the Development of Pulmonary Microvascular Dysfunction in HFpEF. The FASEB Journal, 35.

Duong, C. N., Nottebaum, A. F., Butz, S., Volkery, S., Zeuschner, D., Stehling, M., & Vestweber, D. (2020). Interference with ESAM (endothelial cell-selective adhesion molecule) plus vascular endothelial-cadherin causes immediate lethality and lung-specific blood coagulation. Arteriosclerosis, Thrombosis, and Vascular Biology, 40(2), 378-393.

Ueda, T., Yokota, T., Uno, Y., Mashimo, T., Sudo, T., Ishibashi, T., ... & Kanakura, Y. (2018). ESAM on the Cells of Endothelial Lineage Plays an Important Role in the Development of Definitive Hematopoiesis. Blood, 132, 3848.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant ESAM protein (CAT#: V2LY-0526-LY3962)

Human Recombinant ESAM protein, hFc Tag (CAT#: V2LY-0526-LY3963)

Human Recombinant ESAM protein, His Tag (CAT#: V2LY-0526-LY3964)

Mouse Recombinant ESAM protein, His Tag (CAT#: V2LY-0526-LY8280)

Rat Recombinant ESAM protein, hFc Tag (CAT#: V2LY-0526-LY9371)

Related Products

Mouse Recombinant ESAM protein, His Tag (CAT#: V2LY-0526-LY8280)

Rat Recombinant ESAM protein, hFc Tag (CAT#: V2LY-0526-LY9371)

Human Recombinant ESAM protein (CAT#: V2LY-0526-LY3962)

Mouse Anti-ESAM Recombinant Antibody (E0204) (CAT#: V2LY-0825-LY748)

Rat Anti-ESAM Recombinant Antibody (CBFYE-1219) (CAT#: V2LY-0825-LY750)

Rabbit Anti-ESAM Recombinant Antibody (CBFYE-0063) (CAT#: V2LY-0825-LY749)

Mouse Anti-ESAM Recombinant Antibody (1G8) (CAT#: V2LY-0825-LY757)

Rat Anti-ESAM Recombinant Antibody (CBFYE-1221) (CAT#: V2LY-0825-LY752)

Mouse Anti-ESAM Recombinant Antibody (CBFYE-1220) (CAT#: CBMAB-E1790-FY)

Mouse Anti-ESAM Recombinant Antibody (CBFYE-1224) (CAT#: V2LY-0825-LY755)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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