Mouse Recombinant ESAM protein, His Tag (V2LY-0526-LY8280)

Name: Mouse Recombinant ESAM protein, His Tag
SKU: V2LY-0526-LY8280
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant ESAM protein, His Tag consist of Amino Acid: 1-251 and predicts a molecular mass of 25.6 kDa.

Molecule Mass

25.6 kDa

Sequence

Amino Acid: 1-251

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>92% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tirs, NaCl, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

endothelial cell adhesion molecule

Research Area

Can mediate aggregation most likely through a homophilic molecular interaction.

Biological Process

Bicellular tight junction assembly Source: ARUK-UCL
Cell-cell adhesion Source: ARUK-UCL
Cellular protein localization Source: ARUK-UCL
Homophilic cell adhesion via plasma membrane adhesion molecules Source: Ensembl
Maintenance of blood-brain barrier Source: ARUK-UCL
Regulation of actin cytoskeleton reorganization Source: ARUK-UCL
Regulation of actin filament polymerization Source: ARUK-UCL

Cellular Location

Cell membrane; Adherens junction; Tight junction

Topology

Extracellular: 30-248
Helical: 249-269
Cytoplasmic: 270-390

Hansmeier, N. R., Büschlen, I. S., Behncke, R. Y., Ulferts, S., Bisoendial, R., & Hägerling, R. (2022). 3D Visualization of Human Blood Vascular Networks Using Single-Domain Antibodies Directed against Endothelial Cell-Selective Adhesion Molecule (ESAM). International journal of molecular sciences, 23(8), 4369.

Buncha, V., Fopiano, K. A., Tian, Y., & Bagi, Z. (2021). Role of Endothelial Cell Specific Adhesion Molecule in the Development of Pulmonary Microvascular Dysfunction in HFpEF. The FASEB Journal, 35.

Duong, C. N., Nottebaum, A. F., Butz, S., Volkery, S., Zeuschner, D., Stehling, M., & Vestweber, D. (2020). Interference with ESAM (endothelial cell-selective adhesion molecule) plus vascular endothelial-cadherin causes immediate lethality and lung-specific blood coagulation. Arteriosclerosis, Thrombosis, and Vascular Biology, 40(2), 378-393.

Ueda, T., Yokota, T., Uno, Y., Mashimo, T., Sudo, T., Ishibashi, T., ... & Kanakura, Y. (2018). ESAM on the Cells of Endothelial Lineage Plays an Important Role in the Development of Definitive Hematopoiesis. Blood, 132, 3848.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant ESAM protein (CAT#: V2LY-0526-LY3962)

Human Recombinant ESAM protein, hFc Tag (CAT#: V2LY-0526-LY3963)

Human Recombinant ESAM protein, His Tag (CAT#: V2LY-0526-LY3964)

Rat Recombinant ESAM protein, hFc Tag (CAT#: V2LY-0526-LY9371)

Related Products

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Rabbit Anti-ESAM Recombinant Antibody (CBFYE-1223) (CAT#: CBMAB-E1793-FY)

Human Recombinant ESAM protein, hFc Tag (CAT#: V2LY-0526-LY3963)

Mouse Anti-ESAM Recombinant Antibody (CBFYE-1220) (CAT#: CBMAB-E1790-FY)

Mouse Anti-ESAM Recombinant Antibody (E0204) (CAT#: V2LY-0825-LY748)

Human Recombinant ESAM protein (CAT#: V2LY-0526-LY3962)

Human Recombinant ESAM protein, His Tag (CAT#: V2LY-0526-LY3964)

Mouse Anti-ESAM Recombinant Antibody (1G8) (CAT#: V2LY-0825-LY757)

Rat Anti-ESAM Recombinant Antibody (CBFYE-1219) (CAT#: V2LY-0825-LY750)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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