Mouse Lgals8 ELISA Kit (V2LY-0626-LY1202)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0056 ng/mL
Detection Range
0.01-2 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
36.2 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
lectin, galactoside-binding, soluble, 8
Function
Beta-galactoside-binding lectin that acts as a sensor of membrane damage caused by infection and restricts the proliferation of infecting pathogens by targeting them for autophagy (PubMed:22246324, PubMed:28077878).
Detects membrane rupture by binding beta-galactoside ligands located on the lumenal side of the endosome membrane; these ligands becoming exposed to the cytoplasm following rupture (PubMed:22246324, PubMed:28077878).
Restricts infection by initiating autophagy via interaction with CALCOCO2/NDP52 (PubMed:22246324, PubMed:28077878).
Required to restrict infection of bacterial invasion such as S.typhimurium (PubMed:22246324).
Also required to restrict infection of Picornaviridae viruses (PubMed:28077878).
Has a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans (PubMed:21288902).
Biological Process
Cellular response to virusManual Assertion Based On ExperimentIMP:UniProtKB
Lymphatic endothelial cell migrationManual Assertion Based On ExperimentIDA:UniProtKB
XenophagyManual Assertion Based On ExperimentIMP:UniProtKB
Cellular Location
Cytoplasmic vesicle
Cytoplasm, cytosol

Zhong, J., & Wang, B. (2023). Long noncoding RNA LGALS8-AS1 promotes angiogenesis and brain metastases in non-small cell lung cancer. Acta Biochimica Polonica, 70(3), 551-559.

Wang, G., Shen, K., Xiao, J., Fan, H., Wang, Y., Liu, K., ... & Yang, L. (2023). Long non‐coding RNA LGALS8‐AS1 facilitates PLAGL2‐mediated malignant phenotypes in gastric cancer. The Journal of Gene Medicine, e3487.

Zhai, D., Li, T., Ye, R., Bi, J., Kuang, X., Shi, Y., ... & Lin, Y. (2021). LncRNA LGALS8-AS1 promotes breast cancer metastasis through miR-125b-5p/SOX12 feedback regulatory network. Frontiers in Oncology, 11, 711684.

Zhang, J., Qu, Y., Hou, T., Ge, W., & Zhang, S. (2021). ceRNA Network Analysis Shows That lncRNA LGALS8-AS1 Upregulates DCTPP1 To Promote Breast Cancer Progression Through Sponge miR-125b-5p.

Herkt, C. E., Caffrey, B. E., Surmann, K., Blankenburg, S., Gesell Salazar, M., Jung, A. L., ... & Schmeck, B. (2020). A microRNA network controls Legionella pneumophila replication in human macrophages via LGALS8 and MX1. MBio, 11(2), 10-1128.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant LGALS8 protein, GST Tag (CAT#: V2LY-0526-LY4250)

Human Recombinant LGALS8 protein (CAT#: V2LY-0526-LY4251)

Rat Recombinant LGALS8 protein, GST Tag (CAT#: V2LY-0526-LY9390)

Related Products

Mouse Anti-LGALS8 Recombinant Antibody (CBFYA-0199) (CAT#: CBMAB-X0280-FY)

Mouse Anti-LGALS8 Recombinant Antibody (106.1) (CAT#: CBMAB-G6327-LY)

Mouse Anti-LGALS8 Recombinant Antibody (3E5) (CAT#: CBMAB-L1304-YJ)

Mouse Anti-LGALS8 Recombinant Antibody (CBYCL-276) (CAT#: CBMAB-L0177-YC)

Rat Recombinant LGALS8 protein, GST Tag (CAT#: V2LY-0526-LY9390)

Human Recombinant LGALS8 protein (CAT#: V2LY-0526-LY4251)

Recombinant Mouse Anti-LGALS8 Antibody (CBHb299) (CAT#: CBMAB-Hb503-LY)

Human Recombinant LGALS8 protein, GST Tag (CAT#: V2LY-0526-LY4250)

Mouse Anti-LGALS8 Recombinant Antibody (CBYJL-1533) (CAT#: CBMAB-L1303-YJ)

Mouse Anti-LGALS8 Recombinant Antibody (CBLG1-2376) (CAT#: CBMAB-G6332-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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