Mouse Pgam1 ELISA Kit (V2LY-0626-LY2869)

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Tested Data
Datasheet
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Basic Information

Sensitivity
0.0077 ng/mL
Detection Range
0.015-3 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
28.8 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Phosphoglycerate Mutase 1
Function
Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglyceratea crucial step in glycolysis, by using 2,3-bisphosphoglycerate (PubMed:23653202).
Also catalyzes the interconversion of (2R)-2,3-bisphosphoglycerate and (2R)-3-phospho-glyceroyl phosphate (PubMed:23653202).
Biological Process
Glycolytic processManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of glycolytic processManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of pentose-phosphate shuntManual Assertion Based On ExperimentIDA:UniProtKB
Respiratory burstManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
cytoplasm
cytosol
extracellular exosome
extracellular region
ficolin-1-rich granule lumen
membrane
secretory granule lumen
PTM
Acetylated at Lys-253, Lys-253 and Lys-254 under high glucose condition. Acetylation increases catalytic activity. Under glucose restriction SIRT1 levels dramatically increase and it deacetylates the enzyme.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human PGAM1 ELISA Kit (CAT#: V2LY-0626-LY5024)

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Mouse Anti-PGAM1 Recombinant Antibody (2G1-A6) (CAT#: CBMAB-P1544-YC)

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Rabbit Anti-PGAM1 Recombinant Antibody (
9A5) (CAT#: V2LY-0725-LY1494)

Human PGAM1 ELISA Kit (CAT#: V2LY-0626-LY5024)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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