Mouse Recombinant JAML&AMICA protein, His Tag (V2LY-0526-LY8580)

Name: Mouse Recombinant JAML&AMICA protein, His Tag
SKU: V2LY-0526-LY8580
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant JAML&AMICA protein, His Tag consist of Amino Acid: 1-281 and predicts a molecular mass of 30.9 kDa.

Molecule Mass

30.9 kDa

Verified

HPLC

Sequence

Amino Acid: 1-281

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE.>95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tirs, NaCl, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Junction Adhesion Molecule Like

Function

Transmembrane protein of the plasma membrane of leukocytes that control their migration and activation through interaction with CXADR, a plasma membrane receptor found on adjacent epithelial and endothelial cells. The interaction between both receptors mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, JAML induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair. It also controls the transmigration of leukocytes within epithelial and endothelial tissues through adhesive interactions with epithelial and endothelial CXADR.

Biological Process

Gamma-delta T cell activationISS:UniProtKB
Heterophilic cell-cell adhesion via plasma membrane cell adhesion moleculesManual Assertion Based On ExperimentIDA:UniProtKB
Monocyte extravasationManual Assertion Based On ExperimentIMP:UniProtKB
Neutrophil chemotaxisManual Assertion Based On ExperimentIMP:UniProtKB
Neutrophil extravasationManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of epithelial cell proliferation involved in wound healingISS:UniProtKB

Cellular Location

Cell membrane; Cell junction. Localized at the plasma membrane and enriched in areas of cell-cell contacts (PubMed:12869515).

Topology

Extracellular: 20-275
Helical: 276-296
Cytoplasmic: 297-394

Wu, Q., Wang, Y. B., Che, X. W., Wang, H., & Wang, W. (2023). Junctional adhesion molecule-like protein as a novel target for kaempferol to ameliorate lung adenocarcinoma. Journal of Integrative Medicine, 21(3), 268-276.

Wu, Q., Li, R., Wang, Q. X., Zhang, M. Y., Liu, T. T., & Qu, Y. Q. (2022). Junctional adhesion molecule-like protein promotes tumor progression via the Wnt/β-catenin signaling pathway in lung adenocarcinoma. Journal of Translational Medicine, 20(1), 1-17.

Fang, Y., Yang, J., Zu, G., Cong, C., Liu, S., Xue, F., ... & Sun, M. (2021). Junctional adhesion molecule-like protein promotes tumor progression and metastasis via p38 signaling pathway in gastric cancer. Frontiers in Oncology, 11, 565676.

Sun, Y., Guan, J., Hou, Y., Xue, F., Huang, W., Zhang, W., ... & Yang, J. (2019). Silencing of junctional adhesion molecule-like protein attenuates atherogenesis and enhances plaque stability in apoe−/− mice. Clinical Science, 133(11), 1215-1228.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Recombinant JAML&AMICA protein, hFc Tag (CAT#: V2LY-0526-LY8579)

Human Recombinant JAML protein, His Tag (CAT#: V2LY-0526-LY5108)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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