Rat Trim63 ELISA Kit (V2LY-0626-LY2199)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.0076 ng/mL
Detection Range
0.015-3 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Rat
Assay Type
Sandwich
Reactivity
Rat
Assay Time
1.5 h
Molecule Mass
39.7 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
TRIM63 Gene(Protein Coding) Tripartite Motif Containing 63
Function
E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is catabolized to provide other organs with amino acids. Inhibits de novo skeletal muscle protein synthesis under amino acid starvation. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.
Biological Process
Biological Process muscle contraction Source:Ensembl
Biological Process negative regulation of cardiac muscle hypertrophy Source:Ensembl
Biological Process protein ubiquitination Source:GO_Central1 Publication
Biological Process response to electrical stimulus involved in regulation of muscle adaptation Source:UniProtKB
Biological Process response to glucocorticoid Source:Ensembl
Biological Process response to interleukin-1 Source:Ensembl
Biological Process signal transduction Source:UniProtKB1 Publication
Biological Process skeletal muscle atrophy Source:Ensembl
Cellular Location
Cytoplasm
Nucleus
Cytoplasm, myofibril, sarcomere, M line
Cytoplasm, myofibril, sarcomere, Z line
Colocalizes with TNNI3 in myocytes (By similarity).
Localizes to the M- and Z-lines in skeletal muscle
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human TRIM63 ELISA Kit (CAT#: V2LY-0626-LY4523)

TRIM63 Matched Antibody Pair (1280) (CAT#: APMAB-1280LY)

Antibody Pairs

TRIM63 Matched Antibody Pair (1280) (CAT#: APMAB-1280LY)

Related Products

Mouse Anti-TRIM63 Recombinant Antibody (CBFYM-3169) (CAT#: CBMAB-M3366-FY)

Mouse Anti-TRIM63 Recombinant Antibody (CBT4674) (CAT#: V2LY-0625-LY200)

Mouse Anti-TRIM63 Recombinant Antibody (6G6) (CAT#: CBMAB-T4368-YJ)

Mouse Anti-TRIM63 Recombinant Antibody (CBT2617) (CAT#: V2LY-0625-LY180)

Mouse Anti-TRIM63 (AA 293-304) Recombinant Antibody (6H6D4) (CAT#: CBMAB-M0237-FY)

TRIM63 Matched Antibody Pair (1280) (CAT#: APMAB-1280LY)

Mouse Anti-TRIM63 (AA 293-304) Recombinant Antibody (CBFYM-0207) (CAT#: CBMAB-M0249-FY)

Mouse Anti-TRIM63 Recombinant Antibody (CBYJT-4825) (CAT#: CBMAB-T4367-YJ)

Human TRIM63 ELISA Kit (CAT#: V2LY-0626-LY4523)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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